Effects Of Diet Supplemented With Cinnamaldehyde On Growth Performance,digestive Metabolism And Blood Indicators In Fattening Lambs

The objective of this study was conducted to determine the effects of dietary supplemented with cinnamaldehyde(CA)on growth performance,nutrient apparent digestibility,rumen fermentation and blood indicators of lambs.Nylon-bag technique and in vitro rumen fermentation cumulative gas production method were used to evaluate the rumen degradation characteristics of nutrients and rumen fermentation parameters in vitro with CA at different supplemental levels 0(CON),200(200CA),300(300CA),400(400CA)mg/kg.Then,forty-eight 4-month-old Dorper×thin-tailed Han hybrid male lambs with similar body weight were randomly divided into four groups(CON,200 CA,300CA,400CA)for feeding test and digestion and metabolism experiments.The results were as follows:1)Compared with the control group,the rumen effective degradation rate of acid detergent fiber(ADF),neutral detergent fiber(NDF)and dry matter(DM)in 200 CA,300CA,400 CA groups significantly increased(P<0.05).The effective degradation rate of crude protein(CP)in 200 CA,300CA groups were higher than those in control group(P<0.05),and the400 CA group was lower(P<0.05);There were not significantly affect in total gas volume,p H and MCP among treatments(P>0.05);Compared with the control group,the methane production and NH3-N concentration were significantly reduced by CA added(P<0.05);The contents of total volatile fatty acids,butyric acid,isobutyric acid,pentanoic acid and isovalerate were not significant affect by CA supplementation(P>0.05);Compared with the control group,the content of propionic acid in 300 CA group was significantly increased(P<0.05).2)The ADFI of all groups was not affected by CA(P>0.05),the ADG of lambs in 300 CA group was significantly higher than control group(P<0.05),200 CA and 300 CA groups does decreased F/G(P<0.05);The apparent digestibility of dry matter(DM),organic matter(OM),neutral detergent fiber(NDF)and acid detergent fiber(ADF)of 300 CA group were significantly higher than that of control group(P<0.05);There was no significant difference in gross energy intake among groups(P>0.05),the fecal energy of 300 CA group was significantly lower than that of control group(P<0.05),the digestible energy and apparent digestibility of gross energy of three CA addition groups were significantly higher than those of control group(P<0.05);There were no significant differences in nitrogen intake and urinary nitrogen among groups(P>0.05),compared with the control group,the retained nitrogen,nitrogen deposition rate and nitrogen apparent digestibility of 300 CA group were significantly increased(P<0.05).3)The excretion of uric acid of three CA addition groups was significantly higher than that of control group(P<0.05),compared with the control group,the excretions of xanthine+ hypoxanthine and total purine derivatives of 300 CA group were significantly increase(P<0.05),the synthetic amount of rumen microbial protein of 200 CA and 300 CA groups was significantly higher than that of control group(P<0.05).4)Rumen propionic acid content and total volatile fatty acid content in 300 CA and 400 CA groups were significantly higher than those in control group(P<0.05),the content of butyric acid in 200 CA,300CA and 400 CA groups was significantly higher than that in control group(P<0.05),the contents of acetic acid,valeric acid and A/P of all tests were not significantly affected by CA(P>0.05);There were no significant differences in the activities of β-glucosidase,carboxymethyl cellulase,pectinase and protease in rumen(P>0.05),Xylanase activity in 300 CA and 400 CA groups were significantly higher than that in the control group(P<0.05),the amylase activity in 200 CA and 300 CA groups were significantly higher than that in the control group(P<0.05),the relative number of R.flavefaciens in 300 CA and 400 CA groups were significantly higher than that in the control group(P<0.05),the relative number of P.ruminicola and rumen protozoa in 200 CA,300CA and 400 CA groups were significantly lower than that in the control group(P<0.05),the relative number of methanogens in 300 CA and 400 CA groups were significantly lower than that in control group(P<0.05),and there were no significant differences in the numbers of Protozoan,F.succinogenes,B.fibrisolvens and R.amylophilus of all treatments(P>0.05).5)There were no significant differences in serum Ig A,Ig M,C3 and C4 contents(P>0.05),the activity of LZM and the contents of Ig G in 200 CA,300CA and 400 CA groups were significantly higher than that of control group(P<0.05),the lambs in 300 CA group of TNF-α had significantly lower than control group(P<0.05),the contents of IL-6 in 200 CA,400CA groups had significantly lower than control group(P<0.05);compared with the control group,the activity of GSH-Px in serum of 300 CA,400CA was significantly increased(P<0.05),the activity of CAT in 200 CA,300CA,400 CA groups were significantly higher than that of control group(P<0.05),there was no significant difference in the activities of TSOD and T-AOC(P>0.05).In conclusion,diet supplemented with appropriate amount of CA can improve the rumen degradation rate of nutrients,and increase the daily gain of the lamb,reduce the values of F/G,promote the digestion and utilization of nutrients in diets,improve the function of the rumen fermentation of the lamb,and CA can improve the body’s immunity and anti-oxidation ability,promote healthy lamb growth.Which suggests that CA can be used as a natural and green feed additive in the production of fattening lambs.Under the conditions of this experiment,the recommended dietary supplementation level of CA is 300mg/kg.