Effects Of Probiotics On Growth Performance,Digestion And Absorption Ability,Immune Function And Intestinal Microecology Of Weaned Lambs

In this trial,weaned lambs were selected as the research objective to investigate the effect of Clostridium butyricum on the growth performance,serum biochemical parameters,redox indicators,immune function,pancreatic digestive enzyme activities,rumen fermentation parameters and intestinal microflora of weaned lambs,by feeing the diet with different doses of Clostridium butyricum.Fifteen 3 months old weaned lambs were randomly chosen according to the same body weight and randomly divided into the control group,the low-dose Clostridium butyricum group,and the high-dose Clostridium butyricum group.Five lambs in each group that were fed for 4 weeks.The results obtained indicated the following:1.In the fourth week of the trial,compared with the control group,the average daily weight gain in the high-dose Clostridium butyricum group was significantly increased(P<0.05),and the average daily feed intake decreased significantly(P<0.05).The average daily coarse feed intake in the low-dose Clostridium butyricum group was significantly reduced(P<0.05).2.In the fourth week of the trial,compared with the control group,the UREA content in the high-dose Clostridium butyricum group was significantly reduced(P<0.05).The TG content in the low-dose Clostridium butyricum group increased significantly(P<0.05).3.In the first ?second?third and fourth week of the trial,compared with the control group,the level of serum T-AOC,SOD and GSH-Px activities in the low-dose Clostridium butyricum group and the high-dose Clostridium butyricum group were significantly increased(P<0.01),the MDA content was reduced.4.In the first ?second?third and fourth week of the trial,compared with the control group,the content of serum IL-2,Ig A,Ig G,Ig M and IFN-? in the low-dose Clostridium butyricum group and the high-dose Clostridium butyricum group were increases.The content of serum of IL-1,IL-6 and TNF-? were decrease.5.Compared with the control group,the spleen organ indexes,the pancreas lipase content and the propionic acid in the rumen fluid in the low-dose Clostridium butyricum group was increased(P<0.05),the propionic acid in the rumen fluid in the high-dose Clostridium butyricum group was significantly increased(P<0.01).6.Compared with the control group,the relative expression levels of intestinal barrier function proteins ZO-1 and Claudin-1 m RNA in the low-dose Clostridium butyricum group and the high-dose Clostridium butyricum group were significantly increased(P<0.01).7.Compared with the control group,the relative abundance of Eubacterium and Defiviitalea in the low-dose Clostridium butyricum group were significantly increased;the relative abundance of Ruminococcaceae,Ruminococcus and Porphyromonadaceae,Parabacteroides,the relative abundance of bacteria significantly reduced,the relative abundance of Clostridium,Erysipelotrichaceae,Erysipelotrichi,Erysipelotrichales,Clostridiaceae,Eubacterium in the high-dose Clostridium butyricum group were significantly increased;the relative abundance of Bacteroides,Porphyromonadaceae,Parabacteroides,Akkermansia,Verrucomicrobia,Verrucomicrobiae,Verrucomicrobiales,Verrucomicrobiaceae were significantly reduced.Compared with the low-dose Clostridium butyricum group,the relative abundance of Firmicutes,Clostridia,Clostridiales and Clostridium the high-dose Clostridium butyricum group were significantly increased,the relative abundance of Proteobacteria was significantly decreased.These results suggest that different doses of Clostridium butyricum supplenmented in the feed could enhance the growth performance,antioxidant capacity,immune function and digestive and absorption capacity of the gastrointestinal,maintain good development of gastrointestinal tract and improve intestinal flora.