Mechanism Of GSL Regulating The Callose Deposition On PD During Soybean Resistance To SMV

Soybean is recognized as a high protein and high residue in the world.Soybean mosaic virus disease caused by SMV is one of the common diseases that cause soybean yield reduction.The preliminary work proved that in an incompatible combination composed of soybean variety ’Jidou 7’ and SMV N3,SMV infection will cause the production of NO signaling molecules,and induce calloses to deposit on PD,blocking SMV transport between cells.Callose is strictly regulated by GSLs and BGs,which GSLs plays a key role in soybean resistance to SMV is the focus of this study.Bioinformatics,RT-qPCR,VIGS,prokaryotic expression,EMSA,and callose fluorescence observation and other technologies have systematically studied the regulation of callose synthase and its transcription factors in the process of soybean resistance to SMV.The main results are as follows:1.Using bioinformatics methods combined with the existing transcriptome database of soybean and SMV interaction in this research group,it was identified that the callose synthase Glyma.08G308200(GmGSL07c)and Glyma.18G300200(GmGSL12b)may be the key enzyme that regulates the deposition of callose on PD during soybean resistance to SMV.2.Silencing the two GSLs alone or both together reduced the production of callose and promoted the transport of SMV in soybeans.SMV CP gene could be detected in the uninoculated upper leaves of gene silencing plants,and showed mosaic symptoms infected with SMV.3.Use bioinformatics to predict potential transcription factors that can be combined with the upstream sequence of the callose synthase gene promoter,combined with the transcriptome database to identify the transcription factor gene Glyma.04G170100(GmMYB94)that may regulate the expression of the synthase gene GmGSL12b and is regulated by NO;further verified by EMSA technology that the transcription factor can interact with the upstream binding site of the callose synthase gene GmMYB94 in vitro.4.Silencing the transcription factor gene GmMYB94 can obtain similar results to the silencing callose synthase gene,that is,greatly reducing the production of callose and promoting the transport of SMV in soybean.In conclusion,callose synthase GmGSL07c and GmGSL12b are the key enzymes that control callose deposition on PD during the resistance of soybean to SMV infection;the transcription factor GmMYB94 can positively regulate the expression of the callose synthase gene GmGSL12b.