Effects Of Funneliformis Mosseae On Soybean Root Protein And Related Enzyme Activities In Continuously-cropped Soybean

Soybean[Glycine max(L).Merry] is important economic crop,carrying highest nutrition values.Currently,the quality and yield of soybean is reduced due to the phenomenon ofcontinuous-cropped soybean is increasing prominently,which isa combined consequenceamongplant,soil and microbial,in the North.These consequences are attracting widespread concern of researchers including Arbuscular mycorrhizal fungi(AMfungi)mitigates mechanism of continuous cropping obstacles.HN48 was used as the test material.Funneliformis mosseae,which was the dominant AM fungi in continuous cropping soil,was inoculated in replanted soil including 1 year,2 years and 4 years,and not inoculated F.mosseaewas control group.Soybean root rot disease index and soybean biomass were determinedby using morphologicalobservation method and stoving methodin different continuous cropping soil.The protein ofsoybean root wasassayed by using i TRAQ-LC-MS/MS technology in order to understand the effectsof F.mosseae on soybean root proteins.Defense enzymesinthe root and rhizosphere soil enzyme were assayedusing colorimetric determination method.The primary results are as follows:Along with the increasing years of continuous cropping,soybean root rot disease index showed ascendant trend.F.mosseae promoted the growth of soybean,and decreasedthe disease index.Total131 different proteinswere induced by F.mosseae,including 49 up-regulated and 82 down-regulated expressions.These functions of differentially expressed proteins were involved in stress resistance,glucose and energy metabolism,signal transduction,metabolism of amino acids and proteins synthesis and degradation,which were associated with disease prevention and metabolic pathways.The activities of catalase,polyphenol oxidase,peroxidase and phenylalanine ammonia lyase showed an upward trend and the activity of superoxide dismutaseshowed the downward trend with continuous cropping soils extension.Root defense enzyme activities induced by F.mosseae were significantly higher than the control group.It played an important role inresistanceinducedby F.mosseaethat is phenylpropanesmetabolism pathway.Soil enzyme activitieswere gradually decreasedfollowing soybean growth.The activities of urease,catalase,invertase,phosphatase and polyphenoloxidase were increasedafter decreased,and the activity of cellulase wasdecreased with continuous cropping soil extension.Soil enzyme activities increased by F.mosseae were significantly higher than the control group.F.mosseae induces the synthesis of pathogenesis-related proteins and defense enzymes of soybean root,provides soil fertilizer and promotessoybean growth and development.This experiment provides a theoretical basisfor revealing the mechanism of F.mosseaeinhibiting soybean root rot.