Separation And Detection Of Active Alkaloids Based On Surface Molecular Blotting Technology

Alkaloid is an important natural product,which is widely used in the medical field,especially in the field of cancer.A large number of studies have shown that many alkaloids have anticancer effects,such as matrine,vincristine,paclitaxel alkaloids and so on.In addition,matrine also has many pharmacological activities,such as anti arrhythmia,anti-inflammatory and anti-virus.Therefore,the separation and extraction of matrine alkaloids is of great significance for clinical application.The traditional separation methods have many shortcomings,so the development of an efficient and environmentally friendly separation material has high application value for the separation of Sophora flavescens alkaloids.In this paper,based on molecular imprinting technology and magnetic nano materials,molecular imprinted composites with magnetic separation function were applied to the separation and extraction of matrine.The research contents are as follows.（1）Fe₃O₄ magnetic beads were synthesized by solvothermal method,and then the interaction between template molecules,functional monomers,crosslinkers and initiators was used to coat the surface of Fe₃O₄ magnetic beads with a blot layer to prepare Fe₃O₄@SiO₂@MMIP（magnetically imprinted polymers）,and the appearance and structure of the material were characterized by TEM,VSM,FTIR,XRD and other detection methods.By controlling the reaction concentration,reaction temperature,reaction time and other factors,the adsorption performance of the material under the optimal conditions was determined to be 298K,and the adsorption amount under the 10μg/m L standard was 11.32 mg/g.Through the eluent comparison experiment test,it was found that the particle had the highest elution efficiency in the mixture of methanol and acetic acid（9:1）,and after 5 cycles,the adsorption amount of the particle decreased by only 4.2%,and had good reuse performance.Through the two sets of selective comparison experiments,it is concluded that the blotted particles have good selectivity for matrine,and the actual sample solution is detected by high performance liquid chromatography,the effect is good,because the particles are magnetic,and it is easier to achieve solid-liquid separation than the traditional separation method.（2）The synthesis of Fe₃O₄ magnetic microspheres by solvothermal method,in order to overcome the problem of easy agglomeration of Fe₃O₄ nanoparticles,methacrylic acid is grafted to its surface to prepare Fe₃O₄@MAA nanoparticles,under the interaction of template molecules,crosslinkers and initiators,three-dimensional pores of exactly the same shape and size as the template molecules are formed on the surface of the Fe₃O₄@MAA nanoparticles.The morphology,structure,chemical composition and magnetic properties of the material are characterized by XRD,TEM,FTIR,VSM and other methods,and the unique magnetic properties and specific identification characteristics of the Fe₃O₄@MAA@MMIP composite materials are used for rapid separation and extraction of matrine.After the optimization of experimental conditions,the adsorption performance test was carried out under the solution of 298K and 20μg/m L under the optimal conditions,and the maximum adsorption amount of the Fe₃O₄@MAA@MMIP-imprinted particles to matrine was 25.32 mg/g,and the adsorption amount of the particle decreased by only 3.7%after 5 adsorption-desorption experiments,and it was found that the particle only had good recognition of matrine through selective experiments.