Study On Detection Of Triazole Fungicide Residues By Molecular Imprinting And Its Related Technology

Molecular imprinting technology was applied to prepare molecularly imprinted solid phase extraction materials using one or several triazole fungicides as the template molecules by bulk polymerization and in-situ polymerization,and the materials were used to separate and determine triazole fungicide residues in foods and feeds.Molecularly imprinted solid phase extraction technology,compared with general solid phase extraction technology,had obvious advantages such as good separation enrichment effect,strong specificity and repeated use.Chapter One:Background and significance of the research,triazole fungicide and molecular imprinting technology were introducted.Chapter Two:(1)To prepare effectively bitertanol molecularly imprinted polymer,ultraviolet spectroscopy was used to select the optimal functional monomer alpha methylacrylic acid and research the interaction between bitertanol and the functional monomer.The feasibility and validity of the method were verified by Scachard method.And acetonitrile as optimal porogenic agent was selected by ultraviolet spectrum analysis.(2)Bitertanol molecularly imprinted solid phase extraction agent was prepared by bulk polymerization using bitertanol as template molecule,ethylene glycol dimethyl acrylate(EDMA)as crosslinking agent and alpha methylacrylic acid(MAA)as functional monomer.And bitertanol molecularly imprinted solid phase extraction column was prepared and used for separation and detection triazole fungicide residues in foods and feeds.(3)A bitertanol molecularly imprinted solid-phase extraction membrane was prepared by in-situ polymerization for separation and detection of triazole fungicide residues in foods and feeds using bitertanol as template molecule,EDMA as crosslinking agent and MAA as functional monomer.(4)The preparation method of bitertanol solid phase microextraction rod and its largest adsorption capacity were investigated,and the rod was applied to detection of bitertanol in food and feed.Chapter Three:(1)In the process of study on preparation of myclobutanil molecularly imprinted polymer,acrylamide(AM)as the optimal functional monomer and acetonitrile as the optimal porogenic agent was selected by ultraviolet spectroscopy.And ultraviolet spectrum analysis was used to research the interaction between myclobutanil and the functional monomer.The feasibility and validity of the method were verified by Scachard method.(2)Myclobutanil molecularly imprinted solid phase extraction agent was prepared by bulk polymerization using myclobutanil as template molecule,EDMA as crosslinking agent and AM as functional monomer.And myclobutanil molecularly imprinted solid phase extraction column was prepared and used for separation and detection triazole fungicide residues in foods and feeds.(3)A myclobutanil molecularly imprinted solid-phase extraction membrane was prepared by in-situ polymerization using myclobutanil as template molecule,EDMA as crosslinking agent and AM as functional monomer for separation and detection of triazole fungicide residues in food and feed.(4)The preparation method of myclobutanil solid phase microextraction rod and its largest adsorption capacity were investigated,and the rod was applied to detection of myclobutanil in food and feed.Chapter Four:A multi-template molecularly imprinted solid phase extraction agent was prepared by bulk polymerization using bitertanol,myclobutanil and diniconazole as template molecules,EDMA as crosslinking agent and MAA as functional monomer.A method of the preparation one multi-template molecularly imprinted polymer was established by polymerization reaction at one time.And the polymer was applied to the pretreatment of food and feed samples,triazole fungicide residues in samples were determined by high performance liquid chromatography.