Preparation Of PVC-based SERS Imprinted Flexible Composite Membrane For Specific Detection Of Antibiotics

Antibiotics are widely used in the treatment of human and animal diseases.The detection of antibiotics metabolic residues has become a significant research work.In this paper,combining surface-enhanced Raman scattering technology（SERS）and molecular imprinting technology（MIT）,PVC based SERS imprinted flexible composite membrane sensors were prepared,which could realize high sensitivity,specific recognition of antibiotics,good stability,uniformity and circularity.The main research contents are as follows:1.Using ascorbic acid（VC）as reducing agent,silver nanoparticles（AgNPs）were prepared by chemical reduction method,and then the AgNPs/PVC flexible SERS substrate was prepared by solvent casting method by adjusting the amount of AgNPs and porogen（PVP）.Finally,APM-MIMs sensor was prepared by atomic transfer radical polymerization（ATRP）.SEM,XRD,IR and XPS et al were used to characterize the substrates.APM-MIMs showed good sensitivity to norfloxacin solution of 10^-5 M～10^-9 M.Then,the stability,uniformity,circulability and specificity of the sensor were studied.The results showed that APM-MIMs could be used as the sensor to detect norfloxacin solution.2.The nucleophilic substitution reaction was used to replace-Cl on the surface of polyvinyl chloride（PVC）with groups containing-NH₂.The nucleophilic substitution reaction time,temperature and the concentration of ethylenediamine were optimized.The aminated PVC membrance was placed in silver nitrate solution to combine-NH₂ with Ag⁺.In situ reduction reaction was carried out with ascorbic acid as reducing agent,and AgNPs were grown on the surface of PVC membrance.In order to further enable the SERS substrate to have specific recognition ability,ANPM-MIPs was prepared by the imprinting method of electron transfer-atom transfer radical polymerization to obtain the SERS-MIP sensor.The sensitivity,specificity,circulability,stability,uniformity and anti-interference ability of ANPM-MIPs were investigated.The results showed that ANPM-MIPs can be applied to the detection of ciprofloxacin molecules in complex environments.