| Biomaterial hemocompatibility is an essential consideration in developing synthetic blood-contacting substrate surfaces,particularly to help in the prevention of thrombosis,which can ultimately lead to blood vessel occlusion or system failure.The efficiency of implanted devices remains disappointing due to the clot and thrombus development following a cascade of coagulation reactions triggered by the surface.Herein,to create a bioimplant that can be used in healthcare situations,a surface modification strategy has been applied to graft aminated fucoidan(AFU)onto the surface of polydopamine(PDA)coated polycarbonate urethane(PCU)film.Fucoidan is a marine-extracted polysaccharide with anticoagulant and antithrombotic characteristics.However,straightforward amination of fucoidan is feasible because of its heterogenous nature.Thus,a spacer molecule(epichlorohydrin)with an active group is introduced to activate the modified form towards amination.This new technique is beneficial over previously mentioned techniques on the basis of simplicity,reliability,as well as ease to handle.The successful grafting of fucoidan was confirmed by Fourier transform infrared(FTIR)spectroscopy and X-ray photoelectron spectroscopy(XPS).The hydrophilicity of the modified film has improved significantly due to the hydrophilic properties of fucoidan.In addition,the surface morphology and topography of the modified substrate surfaces were remarked by scanning electron microscopy(SEM)and atomic force microscopy(AFM).The results showed that the grafted surface was rougher compared to the blank PCU surface.More importantly,the modified PCU has been tested at in vitro level,which revealed its significantly improved hemocompatibility,and anticoagulant properties compared to blank PCU.These findings strongly suggest that surface modification by fucoidan could offer a viable approach for enhancing the biocompatibility and bioactivity of blood-contacting materials. |
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