| The medical dressing is a medical material used to cover wounds,injuries or other damages.The ideal wound dressing can not only protect the wound from secondary damage,but also inhibit inflammatory reactions,promote wound healing,and monitor the healing process of the wound.Studies have shown that electrical stimulation can promote wound healing by guiding the migration and proliferation of skin cells.Electrical stimulation devices,conductive materials,and photo/piezoelectric materials have been used for electrical stimulation repair therapy of wounds,but these devices and materials require external power sources.Additionally,piezoelectric materials are easily constrained by light sources/deformation,severely limiting the application of electrical stimulation technology in the field of wound repair.Metal micro-batteries can provide a stable source of electrical stimulation energy without the need for an external power source.Integration with medical dressings has brought new opportunities for wireless applications of electrical stimulation in wound repair.Therefore,this study first prepares an electrically active nanofiber dressing by constructing metal micro-batteries on the surface of nanofibers,and then combines the microelectronic device with the prepared electrically active nanofiber dressing to monitor the wound healing process through temperature changes.The specific research content is as follows:(1)Preparation of electrically active dressings with temperature monitoring function for wound: The template-limited magnetron sputtering technology was used to construct annular Ag/Zn microbatteries on the surface of poly(lactic acid)nanofiber membranes(PLA),and electrically active nanofiber dressings(Ag/Zn@PLA)were prepared.The Ag/Zn@PLA dressing was constructed,and scanning electron microscopy(SEM),energy dispersive X-ray spectroscopy(EDS),and thermal gravimetric analysis(TGA)all showed that the Ag/Zn coating was successfully coated onto the surface of PLA nanofibers.TGA analysis showed that the content of Ag/Zn coating was 5.58%.After Zn coating modification,the contact angle of PLA decreased from 130° to 52°,and the maximum tensile strength of Ag/Zn@PLA could reach 4.6 MPa.This indicates that the dressing has excellent thermal performance,wetting performance,and tensile performance,laying a foundation for its use as a dressing substrate.At the same time,the DHT11 temperature sensor was integrated on the Ag/Zn@PLA dressing,and the corresponding circuit diagram was designed for temperature monitoring.(2)The antibacterial and healing properties of electroactive dressings that can monitor wound temperature are studied below:The inhibition rates of Ag/Zn@PLA against Escherichia coli and Staphylococcus aureus were 95% and 97%,respectively.When the Ag/Zn electrode is wetted by the wound exudate,an oxidation-reduction reaction can occur on the surface of the dressing to generate electrical stimulation.In vitro cell scratch experiments showed that the electrical stimulation produced by Ag/Zn@PLA promotes the migration of fibroblasts.The in vivo mouse wound model showed that Ag/Zn@PLA can promote wound healing by promoting wound re-epithelialization,neovascularization,and collagen deposition.On the 6th day,the healing rate of the Ag/Zn@PLA group was 79.6 ± 1.8%,and on the 12 th day,it was 95 ±1.2%.Furthermore,Ag/Zn@PLA dressing shows no toxicity to cells and has good biocompatibility.In summary,this study integrated microelectronic devices with electroactive dressings to prepare an electroactive functional dressing with electrical stimulation antibacterial,promoting healing,and monitor wound healing process functions.it can provide certain guidance and reference for the development of new and efficient healing materials. |
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