Preparation And Research Of Antibacterial Dressing Based On Alginate/forsythiaside Electrospinnin

The complexity of the wound environment makes wound treatment become a tricky issue,therefore,wound management poses great challenges to the medical profession.For a long time,researchers have been exploring wound dressings that can promote wound healing.Traditional wound dressings such as bandages,gauze,and adhesive plasters are helpful but still have certain limitations.During the wound healing process,an open,moist,nutrient-rich wound environment provides favorable conditions for bacterial growth.Bacterial infection can cause wound deterioration,making them difficult to heal,and even leading to tissue necrosis and systemic infection,posing a significant threat to health.Wound dressings containing silver nanoparticles or antibiotics have been widely used to address wound infection,but the cytotoxicity of silver nanoparticles and the development of antibiotic-resistant bacteria due to the overuse of antibiotics have brought new problems.Nanofibrous membranes have high porosity,a high surface area-to-volume ratio,and a structure similar to the extracellular matrix,demonstrating unique advantages in wound repair.Sodium alginate has good biocompatibility and moisture permeability and adhesion resistance to wounds,making it widely used in wound dressing preparation.In this study,the natural antibacterial properties of the herbal extract of Forsythin were utilized,and Forsythin were mixed with sodium alginate nanofiber membranes by electrospinning to prepare a multifunctional wound dressing with good antibacterial,antioxidant,and woundhealing properties.Based on this,a nanofibrous aerogel was prepared and its cell compatibility and antibacterial properties were investigated,proposing the potential of sodium alginate aerogel as a wound dressing to deal with complex wound environments.(1)There have been certain challenges in preparing high-concentration sodium alginate nanofiber membranes by electrospinning.In this study,high-concentration sodium alginate/carbon polymer dots(CA/CPDs)membranes were prepared by adding carbon polymer dots.The successful doping of carbon polymer dots was demonstrated by UV-visible spectrophotometry.Cell compatibility tests,stress-strain tests,contact angle tests,swelling tests,and other tests showed that carbon polymer dots were nontoxic and improved the mechanical properties,hydrophilicity,and moisture absorption of sodium alginate nanofiber membranes.(2)The clearance of bacteria and free radicals plays an important role in wound healing.In this study,a biological dressing with antibacterial and antioxidant properties was prepared by introducing Forsythin(FT).The influence of Forsythin doping on the properties of CA/CPDs nanofiber membranes was explored through scanning electron microscopy,UV-visible spectroscopy,and other tests.Effective Forsythin concentrations were determined through antibacterial and antioxidant tests.The results showed that the CA/CPDs/FT fiber membrane with 0.5% FT had good antibacterial and antioxidant properties.A mouse full-thickness skin defect model showed that the CA/CPDs/FT nanofiber membrane effectively prevented bacterial invasion,cleared free radicals,and promoted wound healing.(3)The CA/CPDs/FT fiber membrane was prepared into a nanofibrous aerogel by homogenization and freeze-drying techniques,and a double-layer aerogel was prepared by electrospinning poly-ε-caprolactone(PCL)nanofibers.The influence of the concentration of the CA/CPDs/FT nanofiber membrane on the structure of the aerogel was explored.The aerogel had a fluffy porous structure,good air permeability,moisture permeability,antibacterial and antioxidant properties,and strong pressure resistance,but had certain limitations in compression recovery.The good performance of the PCCF aerogel demonstrated its potential for the treatment of chronic wounds with high exudation.