Design,Synthesis And Application Of Agricultural Bioactivity Of Three Aryl Ether Substituted Furanone Derivatives

Strigolactone(SLs)is a kind of small terpenoids,which is composed of three ring endolipids(ABC ring)connected with a D ring through enol ether bond.It widely exists in plants.It can promote the branching of Arbuscular fungi,inhibit the growth of plant branches,and control the tillering of rice(Oryza sativa).In recent years,SLs have attracted the attention of a large number of scientists because of their significant biological activities in Rice and Arabidopsis plants,and their isolation,synthesis and some biological functions have been explored.In nature,the content of natural Strigolactones is relatively small,the stability is poor,and the structure is complex,so a large number of Strigolactones can not be obtained either by separation or chemical synthesis.In order to further understand SLs,scientists have synthesized a series of analogues,such as GR24,to explore their biological activities.However,due to the long synthetic route and high cost of raw materials and reagents,the further research of researchers has been hindered for a long time.Therefore,it is imperative to design and synthesize a class of analogues with low raw material cost,few reaction steps and simple operation.In this paper,we designed and synthesized new SL analogues to explore their tillering control and antibacterial activities(1)On the basis of previous studies and the previous work of our research group,in the presence of potassium carbonate and acetone,the corresponding new Strigolactone analogues were synthesized by S_N2 reaction using 5-bromo-3-methylfuran-2-(5H)one,5-bromo-4-methylfuran-2(5H)one,3-bromo-dihydrofuran-2(3H)as the synthetic substrate and phenol derivatives.The raw materials of the reaction are simple and easy to obtain,the cost is cheap,and it does not need a lot of manpower and material resources,and the yield is good.A total of 40 new Strigolactone analogues were synthesized.(2)The mycelial growth rate method was used to determine the resistance of 40new Strigolactone analogues to six plant pathogens:Fusarium gramineaum,Thanateaphorus cucumeris,Colletotrichum orbiculare,alternaria leaf spot and Botrytis strawberry,Alternaria solani,were determined.At the concentration of 50μg/m L,some compounds showed significant antibacterial activity.(3)It is an important means to improve rice yield by regulating rice tillering.As a new plant branching inhibitor,Strigolactone is the research direction of rice crop technology development in the future.The results showed that its effect on Rice Tillering was very significant.Therefore,we studied the tillering control activity of the new analogues at the concentration of 50μM.The results showed that there were some compounds with significant or extremely significant activity,such as A7,B11,C1,C7,P<0.05;A1,C3,C8,P<0.01.It is obvious that the new SL analogues have a good inhibitory effect on tiller control of rice.Based on this experimental study,we have laid a solid foundation for the further design and optimization of novel Strigolactone analogues.