| Neuronic acid was an n-9 long-chain monounsaturated fatty acid,a core natural component of nerve tissue and nerve cells,which had functions such as promoting brain development and facilitating the repair and regeneration of damaged nerve tissue.The neurorestorative effects of neuronic acid were currently thought to enhance the organism’s resistance to oxidative stress,modulate inflammatory factors and pathways,and repair nerve fibre myelin sheaths.However,the neuroprotective metabolic mechanism of neuronic acid has not been fully elucidated.Acer truncatum seed oil was rich in neuronic acid at about 5% and was an important source of it.The use of proteomics and metabolomics techniques can resolve the metabolic response of neuronic acid in organisms and provide more insight into the physiological functions and mechanisms of action of neuronic acid.Therefore,this paper focuses on the study metabolism of the neurorepair effects of neuronic acid in Acer truncatum seed oil on C.elegans;the establishment of proteomic and metabolomic data analysis methods;and finally the analysis of the mechanism of neuronic acid on C.elegans neurorepair metabolism at the metabolomic and proteomic levels.The main research contents and conclusions are as follows:1.The effects of Acer truncatum seed oil and other vegetable oils with similar fatty acid composition and content on C.elegans neuromotor behavior were compared.It was found that both peanut oil 1,2 and C.elegans oil could repair the locomotor damage in the model group.Compared to the model group,the Acer truncatum seed oil had the most significant effect with24.8% improvement.Similarly,the metabolic maple oil group improved head bobbing of C.elegans was the most significant with an increase of 37.5%;the effects of different concentrations of neuronic acid on C.elegans were studied,and 1 m M of neuronic acid was identified as the next step in the study through indicators of neuromotor behavior,lifespan effects and lipid accumulation.2.The workflow for data analysis about metabolomic and proteomic mass spectrometry was established based on KNIME,a data analysis platform.This paper evaluated the characteristics of the metabolomic workflow,which by combining MS1 and MS2 mass spectrometry information(filtered at RT and m/z tolerance)could filter out more than 50% of significantly reduced potential compounds and retain more than 90% of correct identifications compared to traditional methods.We also tested the Proteomic workflow,which quantified peptides and proteins by two search engines identifying peptides(OMSSA and X!tandom)and inferred proteins and peptides.3.The metabolic mechanisms of metabolomics and proteomics on neural repair of neuronic acid were resolved using the established methodology using C.elegans as the study target.Neuronic acid was found to regulate motor damage mainly by affecting C.elegans glycerophospholipid metabolism as well as fatty acid metabolism.On the proteomics side,it was found to affect protein translation processes,the TCA cycle,glyoxylate and dicarboxylic acid cycle in C.elegans,by up-regulating α-ketoglutarate dehydrogenase and down-regulating Dihydrolipoyl dehydrogenase,and by increasing the expression of the protein Myosin-4 and calcium reticulum protein,which are responsible for muscle contraction.In terms of gene expression,neuraminic acid was mainly used to alleviate C.elegans locomotor damage caused by oxidative stress by upregulating the antioxidant genes gst-4 and sod-3,and to improve C.elegans longevity by regulating daf-16. |
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