Law Of Conversion And Migration Of Oryzanol In Rice Bran Oil Under Alkaline System

In order to solve the problem of easy loss of oryzanol in rice bran oil under alkaline system during the production of nutritional rice bran oil,the extraction of oryzanol from rice bran oil and its application.This project investigates the changes in the conversion and migration of oryzanol and its four main components in rice bran oil under the alkali solution system and alkaline methanol system,studies the conversion and migration law of oryzanol in rice bran oil under alkaline system,and compares and analyzes the difference in the conversion and migration of oryzanol and its components.This thesis is expected to provide the theoretical guidance for the extraction of oryzanol from rice bran oil and its development and application in food,medicine,cosmetics and other fields.Firstly,the HPLC method for the determination of oryzanol in rice bran oil and its soap stock was optimized.The rice bran oil soap stock was acidified and pretreated,and the four main components of oryzanol were separated by methanol:acetonitrile=1:1(v/v)as the mobile phase.The standard curve equations of CAFA,24MCAFA,Camp FA and Sito FA were y=17274x-11691,y=17359x-8938,y=17489x-16407,y=17489x-16407,y=16980x-209,and R~2were greater than 0.999.The precision,sample repeatability,sample stability and dosing recovery of the instrument were verified,and the RSD value was less than 5%.These results showed that the measurement results of the detection method were accurate,the error was small,and the repeatability and stability were good,which could be used for the quantitative analysis of oryzanol in rice bran oil and its soap stock.Secondly,the conversion and migration of oryzanol and its main components in rice bran oil under the lye system were studied.The results showed that under the lye system,the conversion rate of oryzanol and its main components in rice bran oil showed an increasing trend with the increase of reaction time,temperature and alkali added.The lowest conversion rate of oryzanol was 1.47%when the alkali amount was 50%,the temperature was 30℃and the reaction was 20 min.In the lye system,the mobility of oryzanol and its four main components in rice bran oil decreased with the increase of time and temperature,and increased with the increase of alkali added.However,when the temperature was lower than 60℃,the mobility of oryzanol in rice bran oil increased first and then changed in a narrow range with the increase of time.The maximum mobility of oryzanol was 92.07%at 150%theoretical alkali addition,30℃and 40 min reaction time.Under different conditions,the conversion rate of the four main components in oryzanol was basically Sito FA>Camp FA>24MCAFA>CAFA,which indicated that the chemical stability of Sito FA was the worst in the lye system.The order of mobility of the four main components in oryzanol from high to low was basically Camp FA>Sito FA>CAFA>24MCAFA.However,when the temperature was lower than40°C and the alkali amount was 100%and 150%,there was no obvious difference between the mobility of each component.Finally,the conversion and migration of oryzanol and its main components in rice bran oil under alkaline methanol system were studied.The results showed that under the alkaline methanol system,the conversion rate of oryzanol and its main components in rice bran oil showed an increasing trend with the increase of reaction time,temperature and alkali added,but when the alkali amount was 50%,the conversion rate showed increased first and then changed in a narrow range with the increase of time.The lowest conversion rate of oryzanol was 5.13%under the conditions of 50%theoretical alkali amout and 30°C for 20 min.The mobility of oryzanol and its four main components in rice bran oil decreased with the increase of temperature and time,and the mobility increased with the increase of alkali added,but the mobility only changed within a narrow range when alkali amount was 50%.Under different conditions,the conversion rate of the four main components in oryzanol was basically Sito FA>Camp FA>24MCAFA>CAFA,which indicates that Sito FA has the worst chemical stability under the alkaline methanol system.And the mobility of the four main components in oryzanol from high to low was basically CAFA>24MCAFA>Camp FA>Sito FA.