Detection Research Of Root Hypochlorite And Copper Ions By A Fluorescent Probe Containing A Pyridine Schiff Base Structure

At present,the study of fluorescent probes based on organic compounds has very rich experience,for all kinds of fluorescent probe detection mechanism has been quite thorough research,and contains the Schiff base structure has also been widely used,fluorescent probes containing Schiff base structure in cell imaging and detection of various ions,organic matter,compounds,deep attainments,but in the cost,selectivity and detection limit have room for improvement,according to the above problems,this paper designed and synthesized several pyridine Schiff base structure Cu²⁺and Cl O^-fluorescent probes.The methoxyaldehyde was first synthesized from 6-methoxy-1-naphthalene ketone,and the methoxyaldehyde reacted with 2-hyalidine under certain conditions,purified the product with probe NR-1,and conducted a series of spectral tests on the probe NR-1.The test results showed that the probe did not achieve the desired effect,and could not specifically recognize either Cu²⁺or Cl O^-.Improve the experimental scheme,replace methoxyaldehyde with 6-methoxy-2-naphthalene formaldehyde,let it react with 2-hydrazine pyridine,the fluorescent probe NR-2 containing the pyridine Schiff base structure was obtained.resulting in the fluorescent probe NR-2 containing pyridine Schiff base structure.Then,they were tested spectroically,and the results showed that the probe exhibited a double-ion response pattern to both Cu²⁺and Cl O^-.The probe NR-2 was more stable under neutral conditions,it showed excellent interference against some other common ions when detecting Cu²⁺and Cl O^-.By the3-fold signal to noise ratio formula,the detection limit of probe NR-2 for Cu²⁺is6.49×10^-7M,and the detection limit of probe NR-2 for Cl O^-is 6.19×10^-7M.Probe NR-2 has an extremely fast response to Cu²⁺and Cl O^-,Probe NR-2 and Cu²⁺may form a stoichiometry of 4:1 complex of[（NR-2）₄-Cu²⁺].Then cyclohexanone was used for the reaction to obtain cyclohexanone aldehyde,methyl osyl was obtained after recrystallization of the product of iodomethane and2,3,3-trimethyl-3H-Idylthe then react cyclohexanone aldehyde with methyl indor to obtain the product H-Cy-Cl,probes CN-1 and CN-2 were generated by reaction with H-Cy-Cl and cyclohexanone aldehyde,and the p H response,ion selectivity,anti-interference,detection limit for target ion,dynamic change between response and time,and complexation ratio were tested.respectively,ion selectivity,anti-interference,detection limit of target ions,dynamic change relationship between response and time,and complexation ratio were tested.The results show the p Ka value for the probe CN-1 is 6.517±0.14（Abs）and 6.160±0.10（FI）,the p Ka value for the probe CN-2 is 5.65±0.11（Abs）and 5.97±0.08（FI）.The addition of Cl O-causes a quenching effect of the probe CN-1,the probe CN-1 gradually became lighter with increasing Cl O-concentration,and its maximum absorption value and the maximum fluorescence value are gradually decreased.Probe CN-1 showed good selectivity and low O-detection limit and good anti-interference power.Probe CN-2exhibits a two-ion response mode to Cu²⁺and Cl O^-,moreover,the accurate and rapid detection of Cu²⁺and Cl O^-can be achieved in the interference environment,Probe CN-2 and Cu²⁺generated a stoichiometric complex of 1:1.In conclusion,this paper designed a variety of Schiff base fluorescent probes containing pyridine hydrazine structure.This series can achieve double response to hypochlorite and copper ions in interference environment,which is expected to realize the detection and tracking of hypochlorite and copper ions in the environment under heavy metal ion stress.