Preparation Of MXene Nano Flower And Its Application In Exosome Detection

As a 2D transition metal carbide/nitride,MXene material is widely used in the field of biodetection due to its large specific area,good hydrophilicity,abundant surface functional groups,strong adsorption capacity and good biocompatibility.Surface-enhanced Raman scattering（SERS）technology,as a powerful optical technique for trace molecule analysis,has the advantages of high sensitivity,strong specificity,and strong spectral multiplexing ability.The preparation of SERS substrates has always been the focus of research in this field.The excellent electrical and optical properties of MXenes make them promising for the construction of highly sensitive SERS substrates.In this paper,a preparation method of Ti₃C₂ MXene flower-like structure nanoparticles was proposed,and a novel,rapid and highly sensitive exosome detection method was proposed based on the MXene nanoflowers,and its performance as a SERS substrate was explored.Firstly,two synthetic routes of MXene nanoflowers were designed.On this basis,the parameters of each step in the preparation process were adjusted and optimized,and a nanoparticle material with a specific structure with potential biological detection application value was obtained.The origin of the flower-like structure was also preliminarily analyzed.Then,the SERS activity of Ti₃C₂ MXene nanoflowers was initially explored.The Ti₃C₂MXene nanoflower/metal composite SERS substrate was prepared,and the performance of the SERS substrate was characterized using methylene blue dye as a model.The results show that the exosome detection method based on Ti₃C₂ MXene nanoflower substrate has high sensitivity,and the composite SERS substrate based on Ti₃C₂ MXene nanoflower has good SERS activity.The potential application value of this nanomaterial in the field of biological detection is proved.