Preparation Of Functional Inverse Opal And Their Biomedical Application

Due to their unique micro/nano structure and the properties of structural color,photonic crystals(Ph Cs)have shown excellent charm in many fields,such as PBG-PCF,patterned printing,optical sensors,functional coatings,structural color patches,and so on.As a part of Ph Cs,inverse opal hydrogel has attracted numerous researches in filed like biosensors,cell culture,and drug-controlled release.Compared with the Ph Cs template,the anti-structure possesses a larger operating space because of the selectivity of materials.Attributed to the high void ratio,it is easy to cause changes in refractive index when filled with polymers,liquids or drugs,resulting in structural color shift.However,the current inverse opal hydrogel microbeads mostly lack intelligence or only have a single function.In this work,through the combination of smart hydrogel and opal template,multifunctional inverse opal that can be modified and have photothermal responsiveness were prepared respectively.Then,these two kinds of microspheres were individually applied to immobilized enzymes and drugs-controlled release,furthermore,their characteristics were explored and studied.Based on this work,the functionalization of inverse opal hydrogel and its various applications have been realized,providing ideas for the development of functionalized inverse opal materials.The specific research content is as follows:(1)Preparation of inverse opal hydrogel microspheres: Microemulsion droplets with uniform scale and high sphericity were prepared by using a microfluidic device.During the evaporation of water,the nanoparticles self-assembled to form a periodic and ordered structure.After the template fully dried,the pre-gel was fully infiltrated around the nanoparticles.After removing the template particles in hydrofluoric acid,functional inverse opal hydrogel microbeads were prepared.(2)Construction of immobilized enzyme system based on inverse opal hydrogel microspheres: Using biocompatible hydrogel to prepare inverse opal materials.Because of the abundant carboxyl functional groups on the surface of the materials and the high specific surface area of inverse opal,this material could efficiently load glucose oxidase(GOD).Through the carboxyl activating process by EDC/NHS,enzymes with amino groups could be immobilized on the surface of inverse opal.Based on the immobilization of enzymes,the reaction products and p H changes during the catalysis process were explored,and the enzymatic properties of immobilized enzymes and free enzymes were compared.Moreover,the application prospects of the above inverse opal immobilized enzymes are further discussed.(3)Research on drugs-controlled release based on inverse opal hydrogel microbeads: Based on the preparation process of inverse opal hydrogel microbeads,inverse opal scaffolds with photothermal responsiveness were constructed and refilled with the bio-gelatin to achieve drug loading.With the combination of photothermal responsive scaffold and reversible phase-changing hydrogel,the bonding ability of microbeads during photothermal-phase change process was further explored.On the other hand,the drug loading and controllable release were also studied.Based on the structural color of inverse opal structure and the flexible change of the refractive index during the drug release process,the monitoring function of the drug release process was also achieved.