Rheological Properties Of Chitosan Hydrogel Mimicking The Rheological Properties Of Whole Blood

Blood rheology testing plays an important role in clinical medicine.In blood rheology detection,blood viscosity is the most important and most basic indicator;blood viscosity is similar to physiological indicators such as body temperature and blood pressure,and is a systematic indicator that reflects the abnormality of the human body and indicates that certain parts of the human body may be affected.disease.However,the quality control materials used for calibrating viscometers are confusing,and the quality control materials are Newtonian fluids,which cannot reflect the non-Newtonian nature of blood.This leads to poor comparability of test results.The standardization of blood viscosity detection has greatly restricted the promotion and application of hemorheology in clinical medicine,and it has become a problem that needs to be solved now.The ability to develop a stable and reliable non-Newtonian fluid blood viscosity quality control material is a crucial step in the standardization of blood viscosity detection in the future.This topic is based on trying a new preparation method of blood viscosity quality control material.From the perspective of red blood cell morphology,particle size,deformation properties and macrorheological properties of whole blood,chitosan hydrogel microspheres are suspended The fluid form mimics the rheological properties of blood.(1)In the experiment,chitosan hydrogel was prepared by emulsification and crosslinking method.The oil-water ratio,stirring speed and mass fraction of chitosan in the preparation parameters were discussed,and a preparation method with a particle size close to red blood cells was obtained.In this experiment,when other conditions are the same,the oil-water ratio is 8:1,the stirring speed is 500(rad/min),and the mass fraction of chitosan is 3%,the particle size of the prepared chitosan hydrogel microspheres is more in line with the erythrocyte particle size.diameter requirements.(2)Use a cell deformer to measure the deformability of red blood cells and chitosan hydrogel microspheres,optimize the preparation process of chitosan microspheres prepared by emulsification cross-linking method,adjust the concentration of chitosan,the concentration of cross-linking agent glutaraldehyde,and the suspension The p H value changes the deformability of chitosan hydrogel microspheres.When the concentration of chitosan is 2.8%,the concentration of cross-linking agent glutaraldehyde is 2%,and the p H of the suspension is 5.0,the deformability of the prepared chitosan microspheres is good.The basic parameters and conditions for the preparation of chitosan in this experiment: the ratio of oil to water is 8:1,the rotation speed is 500(rad/min),the mass fraction of chitosan is 2.8%,and the concentration of cross-linking agent glutaraldehyde is 2%.The microspheres obtained by the preparation method were suspended in a 0.9wt%sodium chloride solution with a p H value of 5.0,which was recorded as the sample suspension.(3)The chitosan hydrogel microsphere suspensions with different dilution ratios were tested on two different viscometers,the capillary viscometer FASCO-3010 and the rotary rheometer MCR-102,and the same dilution ratio was compared with the whole blood viscosity.The detection rules were compared,and the relationship between the rheological properties of the chitosan hydrogel microsphere suspension and the rheological properties of blood was reflected from the perspective of the viscosity-shear rate relationship diagram.The experimental results show that the sample suspensions with different dilution ratios show non-Newtonian characteristics of shear thinning in the detection of different principles.It shows that the rheological properties of the prepared chitosan hydrogel microspheres suspension can reflect the rheological properties of whole blood to a certain extent.(4)In this experiment,two blood viscometers with different principles were used:capillary viscometer FASCO-3010 and rotary rheometer MCR-102 to detect chitosan hydrogel microsphere suspension samples.Once a month,a total of six times the viscosity indoor storage time stability test.According to statistics,the coefficients of variation of the viscosity values of the two instruments are 2.7%-5.2% and 5.5%-22.2%,respectively.The sample suspension showed certain stability in the detection of two different viscometers.The rheological properties of the prepared chitosan hydrogel microsphere suspension are close to the non-Newtonian properties of whole blood both microscopically and macroscopically.The study of chitosan hydrogel microsphere suspension samples provides a different idea for the future development of a nonNewtonian fluid standard for blood viscosity detection quality control.