| Due to its excellent optical properties and good biocompatibility,semiconducting polymer nanoparticles have become the most promising nanometer therapeutic materials.Although the triplet energy of most semiconducting polymers is greater than 0.98 e V(the energy required to initiate singlet oxygen),it is theoretically feasible to perform photodynamic therapy,few therapeutic systems based on semiconducting polymer nanoparticles have been reported.A very important reason is that semiconductor polymer nanoparticles are prepared by a single limited method,mainly microemulsion method and reprecipitation method.These two methods have the disadvantages of uneven size distribution and difficult controllable synthesis.Functionalized modification of semiconductor polymer nanoparticles also relies on complex and time-consuming modification methods of surface functional groups.Therefore,it is of great significance to develop a simple and effective method for the preparation and functionalization of semiconductor polymer fluorescent nanoparticles with uniform size distribution and excellent performance for the exploration of the practical application of this nanomaterial.Flash nanoprecipitation(FNP),which is widely used to fabricate polymer nanomaterials,is a simple and rapid self-assembly technique for preparing nanoparticles.However,the synthesis of semiconductor polymer nanoparticles based on FNP method has not been reported,and the research on functional modification of these nanoparticles is few,and the research on the application of these nanoparticles in the diagnosis and treatment of tumors is also lacking.This paper aims to prepare nanometer diagnostic agent with excellent performance based on semiconductor polymer with excellent photophysical properties,and study its application in tumor diagnosis and treatment.The following three main works have been carried out:(1)A series of semiconductor polymer quantum dots with excellent properties and adjustable particle size were prepared by using FNP technology,and the morphology,particle size and spectral properties of the polymer quantum dots were characterized.The results of cell experiments show that the polymer quantum dots prepared by this method have low biotoxicity and good cell imaging effect.(2)Tumor microenvironment of lack of oxygen and low acidity led semiconductor polymer light dynamics,low treatment efficiency of metal nanoparticles doped is an(3)effective method to improve the efficiency of dynamic treatment of this materials,we adopt FNP technology based on MEH PPV and copper salts as precursors,PS-b-PVP as reactants,step in the preparation of copper oxide functionalization of semiconductor polymer nanocomposites,i.e.Cu_xO@PS-b-PVP@MEH-PPV(x=1 or 2).The results show that the nanomaterial not only fully retains the excellent optical properties of the semiconductor polymer,but the copper oxide nanoparticles in the shell also endows it with strong chemodynamic treatment.(4)In order to further simplify the synthesis steps and improve the efficacy of diagnosis and treatment,we utilized electrostatic and hydrophobic effects to synthesize the near-infrared polymer Poly(cyclopentadithiophene-alt-benzothiadiazole)nanocomposite loaded with copper nanooxides,namely Cu_xO@PCPDTBT(x=1 or 2),using FNP technology in one step.The nanomaterial has strong absorption in the near infrared region,and its triple energy is 1.0 e V.It can be used as both a near infrared fluorescent agent and a photosensitizer.The nanomaterial can not only perform near-infrared imaging,but also perform combined photodynamic therapy and chemotherapy to effectively inhibit tumor growth in xenograft mouse models. |
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