Fabrication And Properties Of PLGA-SF Electrospun Nanofiber Membrane

Due to the controllable fiber morphology,small fiber diameter and excellent physical and chemical properties,electrospun polymer nanofibers membranes have been widely used in the field of biological engineering such as tissue engineering,wound dressing,affinity membrane and drug carrier.In this paper,the monolayer PLGA-SF electrospun fiber membrane,multilayer PLGA-SF electrospun fiber membrane and the monolayer and multilayer drug-loaded electrospun fiber membrane with different PLGA-SF mass ratio were prepared by electrospinning technology,and the structure and properties of the fiber membrane were studied.In this paper,the poly(lactic-co-glycolic acid)(PLGA)-silk fibroin(SF)nanofibers with different mass ratios of 1:1,2:1,4:1 and 10:1 were prepared using electrospinning technology.The micromorphology,chemical structure,hydrophilic property,tensile strength and degradation properties of the electrospun PLGA-SF nanofibers with different with PLGA and SF mass ratios(1:1,2:1,4:1 and 10:1)were characterized.The results showed that electrospun PLGA-SF nanofibers displayed the smallest diameter(86.69±36.13 nm)when the mass ratio was 2:1.Furthermore,this electrospun nanofibers membrane showed good hydrophilic properties,high tensile strength(3.29± 1.25mpa),and high elongation at break(38.66±0.94%).In addition,the speed of degradation presented relatively slow.The multilayer PLGA-SF nanofibers were prepared by electrospinning technology,which was composed of the outer layer with the mass ratio of PLGA to SF 2:1,and the mass ratio of PLGA to SF 1:1,4:1 and 10:1 as interlayer.The micromorphology,hydrophilicity,tensile strength and degradation properties of the electrospun multilayer PLGA-SF nanofibers were characterized.The results showed that the average diameter of electrospun multilayer PLGA-SF nanofibers decreased first and then increased with the increase of interlayer PLGASF mass ratio.When the interlayer PLGA-SF mass ratio was 4:1,the electrospun multilayer PLGA-SF nanofibers displayed the smallest average diameter(161.53±38.46 nm)and the highest tensile strength(3.03±0.30 MPa).However,all the electrospun multilayer nanofibers membranes with different mass ratios of PLGA and SF presented good water absorption,and small protein adsorption capacity as well as good degradation properties.More remarkable,the degradation time of electrospun multilayer nanofibers membrane showed longer than that of electrospun monolayer nanofibers membrane.The acetylsalicylic acid(ASA)was selected as the drug model,and the drug-loaded electrospun monolayer and multilayer PLGA-SF nanofibers membranes were prepared.The drug release rate,blood compatibility and cytocompatibility of these electrospun nanofibers membranes were characterized.The results showed that when the mass ratio of PLGA to SF was 2:1,the drug-loaded electrospun monolayer nanofibers membrane displayed the fast drug release rate,indicating that the drug-loaded electrospun nanofibers membrane could be used for rapid drug release.However,the drug release rate of electrospun multilayer nanofibers membrane with the mass ratio of interlayer PLGA to SF 4:1 was presented relatively gentle,and the drug release time was long,which indicated that electrospun multilayer nanofibers membrane could be used in the field of sustained drug release.Moreover,both drug-loaded electrospun monolayer and multilayer PLGA-SF nanofibers membranes showed good blood compatibility and little cytotoxicity.And the anticoagulant rate met the requirements of biological products.