Construction And Performance Study Of Wound Microenvironment Responsive Protein Hydrogel Drug Delivery Syste

Wound care is one of the most common clinical problems.The body’s circulatory system is exposed due to skin damage and the appropriate microenvironment also increases the risk of bacterial invasion,thus additional antimicrobial protection is required during recovery.The ideal gel wound care materials require antibacterial properties,promote healing,biosafety,and other aspects.To meet these requirements,this study used polyactivated lactynyl ester and bovine serum protein(BSA)as raw materials through amino-alkyne bioconjugate reaction to construct a wound microenvironment-responsive natural protein hydrogel drug delivery system,bFGF and antibiotic gentamicin sulfate(GS)were loaded as model drugs by diffusion,and investigated its physical properties,biological properties and wound healing performance.The main work is as follows:(1)Active polyesters were constructed by polyethylene glycol and butynedioic acid,then by click reaction cross-linking with bovine serum albumin to obtain protein hydrogel(N-gel).Subsequently,the gel formation mechanism was explored by magnetic resonance imaging(MRI),infrared spectroscopy(FTIR),and gel permeation chromatography(GPC).Besides,the rheological properties and swelling properties of the hydrogels were tested,and the results showed that the hydrogels have excellent swelling properties(643 %),meeting the requirements as the drug carriers.(2)The chemical and biological properties of the protein hydrogel drug delivery system(PEBA-gel)were systematically characterized.In vitro antibacterial and degradation experiments of PEBA-gel,the results exhibit that PEBA-gel has splendid antibacterial and enzymatically degradable.The acute hemolysis experiment verified that PEBA-gel had no obvious effect on blood cells.Bacterial acidic metabolites,as well as anaerobic respiratory metabolism of lactic acid in wound wounds,result in an acidic wound tissue microenvironment,with a pH approaching the BSA isoelectric point and rendering it electrically neutral,thereby releasing growth factors.In vitro drug release experiments show that PEBA-gel has responsive drug release ability,while the cell migration and cell proliferation experiments further verify that PEBA-gel promotes cell proliferation and accelerate wound healing.(3)An animal model was established to verify the in vivo application performance of the protein gel.PEBA-gel successfully controlled the deterioration of infection in mice,and promoted cell proliferation and differentiation,thus accelerating wound healing.Besides,the novel hydrogel material shows no significant obvious inflammatory stimulation and other adverse reactions to the vital organs of mice,meeting the application requirements of biomedical healthcare materials.