Preparation Of Multifunctional Self-Assembled Alkylated-Chitosan/Alginate Microcapsules And Evaluation Of Wound Healing

Promoting effective and rapid repairing on actual wound with serious hemorrhage and bacteria invasion remains a critical challenge in clinical wound treatment.Therefore,wound treatment and healing have become a basic and important medical subject in the application of biomedical engineering and pharmaceutical preparations,which attracts more and more attention in recent years.Wound healing is a complex and systemic physiological process involving multiple coordinated stages,such as hemostasis,antiinflammatory,proliferation,and tissue remodling.Therefore,besides excellent biocompatibility and biosafety,ideal therapeutic materials for wound treatment should also be endowed with the synergistic function of promoting the whole process of healing,in order to achieve a systematic,comprehensive and comprehensive wound treatment effect.In this study,based on the physiological process of healing,we report multifunctional selfassembled alkylated chitosan/alginate microcapsules(SAAMs)as novel therapeutic materials for promoting whole-stage wound healing.Conducted by the Schiff base reaction of dodecaldehydes and chitosan,the alkylated chitosan(ACS)with antibacterial and hemostatic properties was synthesized successfully.Based on the phase separation of dextran(DEX)and polyethylene glycol(PEG),the SAAMs are assembled from ACS and alginate(ALG)by microfluidics.The prepared SAAMs possess controllably uniform size and round shape.Moreover,the size and distribution of SAAMs can be easily manipulated by adjusting the flow rate of dispersed phase and the applied voltage intensity.In addition,the emulsion system of DEX and PEG avoid the participation of organic reagents.Thus,the prepared SAAMs have incomparable biocompatibility and biosafety,which contributes to the efficient encapsulation and slow release of bioactive drugs such as proteins.Furthermore,the scanning electron microscopy(SEM)was used to observe the porous internal structure,which benefits the prepared SAAMs to absorb blood and facilitate the hemostatic process.Moreover,in the hemostasis experiment of acute tissue injury,the amount of blood loss and hemostatic time of liver and femoral artery injury treated with SAAMs can be reduced by 80% and 70% compared with the control group.What’s more,the SAAMs exhibit superior antimicrobial properties due to the bacterial anchoring ability of the carbon chain and the bacterial destruction ability of chitosan.Therefore,SAAMs can effectively inhibit bacterial growth,destroy the bacterial physiological structure,and relieve bacterial infection.In the treatment of infected chronic trauma,SAAMs can effectively accelerate wound repairing and granulation tissue generation,reduce inflammatory infiltration,and promote the healing process of wound.In this study,the microfluidic technology is used to fabricate a novel multifunctional self-assembled alkylated chitosan/alginate microcapsule,which exhibits various functions of promoting the wound treatment,such as collaborative hemostatic mechanisms,effective antibacterial activity,high reproducibility,adaptability to complex wound,and efficient encapsulation of drugs.Therefore,SAAMs show superior therapeutic effects over all stages of the wound healing process,representing a significant advance in developing multifunctional therapeutic materials with great promises in wound treatment and related biomedical applications.