Identification Of Potato OPT Gene Family And Functional Analysis Of StYSL12 In Cadmium Toleranc

Conserved oligopeptide transporters（OPTs）in Cadmium（Cd）tolerant potato"Yunshu 505"are induced by Cd stress and have the ability to transport substrates over long distances.However,its selective transport of Cd and its function of isolating and resolving Cd remains unclear.Therefore,in this study,members of potato OPT gene family were identified.The key gene StYSL12 was screened according to 11 biological and other results,and then cell biology and genetics experiments were performed on it to explore the structural characteristics,specific expression pattern,Cd accumulation in eukaryotic cells and tolerance in plant of potato OPT gene family members.The main results are as follows:（1）Based on OPT domain（PF03169）,21 OPT genes were identified by HMMER program in potato.OPT gene is divided into PT and YSL subfamily.The genes of Islam subfamily were named as StYSL1-StYSL12,and those of PT subfamily were St OPT1-St OPT9,collectively known as St OPTs.（2）Special StYSL12 was obtained through comprehensive analysis of St OPTs.StYSL12contains more than 60%of the classical motif in the OPT domain and contains a component related to abiotic stress.StYSL12 is positively upregated more than 50 times under heavy metal stress,indicating that StYSL12 is functively conserved and may play an important role in heavy metal stress.（3）Functional complementarity assay of yeast mutants showed that StYSL12 was a key Cd tolerance gene in cells.Yeast mutant of StYSL12>wild-type yeast>empty carrier yeast mutant.These results suggested that StYSL12 restored the mutant phenotype and increased Cd accumulation in cells.（4）StYSL12 significantly improved the tolerance of Arabidopsis to Cd.After 3 days of100μM Cd Cl₂treatment,the wild-type Arabidopsis was more poisoned by Cd;after 30 days,the leaf phenotype of the transgenic plants was better than that of the wild-type,and the Cd accumulation showed root>leaf>stem>fruit pods,and StYSL12 increased.Cd tolerance in Arabidopsis and increased Cd accumulation in plants.In conclusion,the St OPTs functional gene of potato with tissue-specific cadmium stress stress was excavated in this study,and the key gene StYSL12 was identified to regulate the distribution and tolerance characteristics of Cd in plants,providing a theoretical basis for understanding the tissue-specific distribution and detoxification mechanism of potato cadmium.It also provides important genetic resources for potato safe production and remediation of Cd contaminated soil.