Functional Analysis Of MAT E Gene Anti-metal Stress In Cajanus Cajan

Sustainable forest development and green ecological civilization construction are the current guidelines for forestry development and an important part of forest management.How to better improve the quality of forests and do a good job in the development and basic research of related stress-resistant germplasm is one of the important directions.At present,with the rapid development of modern industrialization,the impact of metal pollution on forest quality has also increased.Metal pollution seriously affects the physiological processes such as growth and development of trees,photosynthesis and the operation of enzyme systems in trees.Metal pollution has become an important factor limiting the quality of forests.As a kind of medicine and food,the pigeonpea is one of the important woody grain and oil resources in China.The soil in the planting area is mostly acidic,and the metal toxicity in acid soil is particularly serious.In plant detoxification mechanisms,MATE protein can eliminate endotoxin or protect against extracellular toxins and play an important role in resisting metal stress.Therefore,in order to study the mechanism of plant resistance to metal stress,this study performed genome-wide analysis and experimental verification of MATE protein in pigeonpea.The conclusions are as follows:1.Identification and bioinformatics analysis of the MATE genes of pigeonpea.A total of 67 MATE genes(CcMATEs)were identified from the pigeonpea genome,and bioinformatics analysis was performed to classify all MATE proteins into three large groups,eight subgroups.Chromosome localization prediction revealed that 51 CcMATEs were located on 8 chromosomes of pigeonpea.Protein motif analysis and three-dimensional protein structure prediction all found that the structure of pigeonpea MATE protein has a certain regularity.2.Tissue specificity and metal stress analysis of MATE genes of pigeonpea.Analysis showed that most of the genes in all eight subgroups were tissue specific.Under three metal stresses(aluminum,manganese and zinc),CcMATE34 and 45 were found to be up-regulated during the three metal stress treatments.3.Preliminary study on the aluminum resistance mechanism of pigeonpea.Three genes,CcMATE32,34 and 45,were selected for further analysis.Using the pigeonpea transient transgenic system,it was found that the expression of the enzyme gene related to citric acid synthesis was improved in the root tip of the three overexpressed genes,especially CcMATE32.At the same time,subcellular localization experiments revealed that they were all localized on the plasma membrane.In summary,members of the MATE gene family in pigeonpea have certain structural conservation and are mostly tissue-specific.In this study,the expression of CcMATEs under metal stress was analyzed,and the role of CcMATE32,34 and 45 in aluminum stress was further explored.These results not only lay the foundation for the in-depth study of the resistance mechanism of pigeonpea against metal stress.More evidence for the development of high-quality metal-resistant varieties of pigeon peas and other plants has opened up ideas for improving the quality of forest plant resources.