Preparation And Application Of Size-controlled PMMA Microspheres Based On Microfluidic Technology

With the development of time and science,microfluidic technology has attracted more and more attention of scientific researchers due to its advantages of high efficiency and low consumption,and its application prospects in biology,medical treatment,environment and other aspects are also very broad.Microfluidic technology to prepare microspheres has become a current research hotspot.Microspheres are widely used in various fields.Among them,polymer microspheres have attracted much attention because of their surface modification,carrying different functional groups and showing functional characteristics.However,traditional preparation methods have certain limitations.The emergence of microfluidic technology to prepare polymer microspheres has opened up new preparation prospects.In addition,fluorescent encoded microspheres have high-throughput and high-sensitivity detection characteristics,and are also widely used in biological analysis such as medical diagnosis,drug analysis,and genetic testing,but how to accurately encode microspheres is always at the bottleneck stage.,The emergence of microfluidic technology has changed this situation.In recent years,the use of microfluidic technology to prepare uniform and monodisperse microspheres has become one of the research hotspots in recent years.Therefore,the main contents and results of this paper are as follows:（1）In this paper,a self-assembled coaxial flow microfluidic platform was built,and P（MMA-St）composite microspheres were prepared by using the coaxial flow microfluidic device,and the effect of P（MMA-St）composite microspheres on methyl violet was investigated.Adsorption properties of dye wastewater.The effects of p H,initial concentration of methyl violet solution,adsorption time and reaction temperature on the adsorption efficiency were investigated.The results show that the adsorption efficiency is as high as 92.59%when the dosage is 50 mg,the p H is 9,the temperature is 30℃,and the concentration of methyl violet solution is 50 mg/L.The adsorption efficiency can still reach more than 80%within 7 times of reuse,the material has good adsorption capacity and can be recycled.（2）Using the constructed self-assembled coaxial flow microfluidic platform,P（MMA-St）/Fe₃O₄magnetic polymer microspheres were prepared and the adsorption performance of P（MMA-St）/Fe₃O₄magnetic polymer microspheres for malachite green dye wastewater was explored.The results show that the magnetic polymer microspheres can quickly and effectively adsorb and separate the cationic dye malachite green in water.The effects of p H,initial concentration of malachite green solution,adsorption time and reaction temperature on the adsorption efficiency were investigated.When the concentration of malachite green solution is50 mg/L,the adsorption efficiency is as high as 94.38%,the adsorption efficiency can still reach more than 80%within 9 times of reuse,and it has good adsorption.ability.（3）Using the built microfluidic chip platform,single-color quantum dot fluorescent-encoded microspheres and multiple-color quantum dot fluorescent-encoded microspheres were prepared.Characterization methods were used to characterize the microspheres,and the stability of the microspheres was evaluated.sex was analyzed.The results show that the quantum dot fluorescent encoded microspheres prepared by this method have no obvious spectral overlap and spectral distortion,and have good stability and no leakage,which once again proves that the platform can be used for the preparation of a variety of encoded microspheres.