Preparation Of Photosensitive TLC Plates For Study On Light-responsive Separation Behaviors

Stimulus-responsive materials can reversibly alter their structure and function according to external physical,chemical,or biological stimuli,providing an ideal choice for novel separation media.Light irradiation is one of the most commonly used external stimuli of light responsive materials because of its convenient application and easy to control.Because light is a convenient and non-invasive signal,the light control mechanism of light responsive materials is widely used in drug recognition,cell signal recognition,light controlled molecular switching and other fields.Azobenzene compounds are one of the most common photoresponsive materials.Azobenzene structure undergo reversible cis-trans configuration under light induction,and the color,molecular size,dipole moment,structure and polarity of the molecule will also change.In this study,azobenzene with photoinduced heterogeneous reversible change was introduced into the stationary phase.Combined with the advantages of convenient TLC manipulation,a thin layer chromatography with photoinduced polarity in situ and reversible control was prepared.The main research contents are as follows:1.4-[3-（triethilanyl）were prepared with different thickness（44.7,72.2,117μm）（SEM）,NMR carbon spectrum(¹³C NMR),X-ray photoelectron spectrum（XPS）,and the relative standard deviation（R_f）of 5.14-16.04%.2.Through the stationary phase polarity evaluation test,azobenzene modified silica gel fixed phase is polar stationary phase,after 365 nm light induced fixed phase polarity increase,after 473 nm light induced stationary phase polarity recovery,this is consistent with the azobenzene molecule cis structure polarity greater than trans,indicating that light induction can achieve stationary phase polarity reversible regulation and good repetition.3.Taking rhodamine B as an example,the chromatographic separation behavior of induced photosensitive LC was evaluated,and the results showed that the maximum change of chromatographic retention behavior can be achieved for 10 min,and reversible regulation of target retention behavior can be achieved.The integration of multiple stationary phases on the same plate and causing a variety of changes in target retention behavior was achieved by changing the light-induced position.4.Under light induction,the separation of various targets,such as multiple dyes,flavonoids isomers,was achieved using photosensitive TLC plates.This study proved that the polarity of stationary phase can be regulated and reversible,to realize light response single stationary phase separation multiple component complex samples provides experimental basis,further prove that can achieve in situ light regulation stationary phase polarity,provide research basis for the development of multidimensional photosensitive liquid chromatography,provide new ideas for solving complex biological sample separation analysis.