Mining And Expression Interaction Analysis Of Key Genes Of Sex Differentiation In Asparagus Taliensis

Plant sex differentiation is a hot research direction in the field of plant reproductive biology.Transcriptome sequencing technology provides a high-throughput,efficient and high-precision method for studying plant sex differentiation.Asparagus taliensis is a species of the genus Asparagus in the early stage of sex differentiation,which is endemic to southwest China.It is an important material for the study of the evolution of Asparagus species,especially the mechanism of sex determination and the origin of sex chromosomes.In this study,transcriptome sequencing technology,flower development or sex differentiation related gene family identification and protein interaction were used to study the sex differentiation of Asparagus taliensis,and its molecular mechanism was preliminarily discussed.The results are as follows:(1).The quality of transcriptome sequencing data was good,and a large number of high-quality cleaning data were obtained after quality control.A total of 135 Gb clean reads were obtained,and the Q30 values of each sample were all above 93 %.A total of 2597 differentially expressed genes were screened in the flower tissue comparison(MFvs FF),a total of 3072 differentially expressed genes were screened in the root tissue comparison(MRvs FR),and a total of 1684 differentially expressed genes were screened in the stem tissue comparison(MSvs FS).Through further screening,1424 differentially expressed genes were found,which were specifically expressed in flowers.These differentially expressed genes may be related to gender differentiation in Asparagus taliensis.Further GO function analysis showed that the highly expressed genes in female flowers were mainly involved in biological processes related to reproduction,such as post-embryonic morphological process,floral organ development,pistil development and auxin.The highly expressed genes in male flowers are mainly involved in biological processes such as pollen development,gamete development,plant development,cell growth,morphogenesis,ion transport and pollination.(2)A total of 73 MADS-box gene family members,136 MYB gene family members,141 b HLH gene family members,and 36 DUF247 gene family members were identified,and the physicochemical properties of their encoded proteins,promoter cis-acting elements,conserved motifs,phylogenetic relationships,and gene expression were analyzed.Most genes are expressed in each sample,and this broad-spectrum expression pattern is consistent with their important regulatory factors for the growth and development of Asparagus taliensis.However,some genes exhibit tissue-specific expression patterns,which are only specifically expressed in flower tissues and play an important role in flower development or sex differentiation.(3).A total of 43 differentially expressed genes that may be involved in the sex differentiation of Asparagus taliensis were screened.These genes are mainly involved in flower development,pollen development,pollen germination and gamete development pathways.(4).We verified the expression patterns of 33 differentially expressed genes.The results showed that the expression levels of these differentially expressed genes were different in different tissues and different plants,indicating that these genes have a stable expression pattern in sex differentiation.In addition,we also found that some differentially expressed genes are closely related to biological processes such as floral organ development,tapetum development,and pollination.These results indicate that these genes play an important role in regulating plant sex differentiation.(5).Through yeast two-hybrid experiments,it was verified that there was a strong interaction between Ata TDF1(Belongs to the MYB gene family member,the gene ID is Ata07G005870)and Ata AMS(Belongs to b HLH gene family member,gene ID is Ata07G029840).It was preliminarily speculated that Ata TDF1 and Ata AMS may form heteropolymers to form transcriptional complex regulatory factors,which play an important role in the process of sex differentiation of Asparagus taliensis.In summary,we used transcriptome sequencing technology to study the sex differentiation of Asparagus taliensis,and found some sex-specific genes and transcription factors,which were involved in the regulation of sex differentiation of Dali Asparagus.These results provide an important reference for further exploring the molecular mechanism of gender differentiation in Asparagus taliensis.