Preliminary Analysis Of Transcriptional Regulatory Pathway Of Tapetum Based On Microarray Data

Normal pollen formation,which plays an important role in plant reproductive process, not only depends on the molecular and cellular function of its own, also depends on the supply of anther, in particular the tapetum cell. Model plant Arabidopsis's tapetum development control by a series of complex gene regulatory networks. Regulatory networks is very important to study the molecular mechanism of tapetum development. As the tapetum works an important role during pollen development, a great number of scientific workers research the tapetum genes and have reported five tapetum transcription factor's function. Preliminary work in our laboratory has also identified five transcription factors' relationships. However, the biological processes of tapetum regulated by DYT1, TDF1, AMS, AtMYB103 are not clear.In this study, we make use of mutants of Arabidopsis DYT1, TDF1, AMS, AtMYB103 to do biochip to produce high-throughput experiment data. We select the gene (mutant/wild-type<0.5) for the downregulated genes. Functional classification of genes will be get by using the theory based on GO and Mapman. The down-regulated genes can be divided in five classes:protein degradation, RNA transcription, cell wall synthesis and degradation, kinase receptor and peptide transport.We found that secondary metabolism is regulated by DYT1, cell activity is regulated by TDF1, lipid metabolism is both regulated by AMS and AtMYB103. DYT1, TDF1, AMS, AtMYB103 common play an role in five five functional modules who are RNA regulation, cell wall degradation, signal kinase receptor, peptide transport during the development of tapetum.