Study On Microsphere Based Large Field Of View Super-resolution Imaging Technology

Optical microscope is a vital tool to explore the microscopic world for humans,which plays an important role in the fields of biology,medicine,materials science,and precision measurement.Due to the diffraction limit,developing super-resolution optical microscopy imaging technology with higher image quality and spatial resolution has become a hot research frontier.Super-resolution imaging technology based on microspheric lens has great development potential because advantages of being easy to implement,simple operation and label-free.However,the field of view(FOV)of a single microsphere is limited.It is difficult to locate the microspheres accurately.Improving the maneuverability of microspheres and expanding the FOV of superresolution imaging have become the key of this technology development.In terms of the controllable positioning and manipulation of microspheres and the expansion of the FOV of microsphere lens super-resolution microscopic imaging,researchers have proposed a variety of solutions.However,there is still a lot of space for the combination of super-resolution image quality and large field imaging.Aiming at the superresolution microscopic imaging of the microsphere lens with a large field of view,the influence of the type,size and medium environment of the microspheres on the imaging quality are discussed.The experimental study of microsphere imaging under the control of optical tweezers is carried out.The main contents are as follows:1.The latest research status of microsphere superresolution technology was reviewed.The control methods of microsphere were summarized in detail,including mechanically contact control,microsphere assembly layer,non-contact control,and microsphere-objective integration.Their technical characteristics were discussed.2.The principle of super-resolution imaging of microspheres is described.The photon nanojet effect and its parameters under different microsphere lenses and media environment are analyzed through simulation.The best combination type is analyzed based on the simulation results.3.Experiments on the super-resolution imaging characteristics with a large FOV were carried out and designed.The effects of microsphere lens and medium environment on super-resolution imaging were analyzed according to the results.The simulation results were verified.The method of optimizing super-resolution image was obtained.The imaging surface was analyzed.The attention points of large-field scanning imaging were proposed.4.The large-field super-resolution scanning imaging of microspheres was explored.Simulation and experiment were designed to explore the feasibility of scanning methods.Aiming at the microsphere matrix scanning,the microsphere matrix under different conditions was simulated.The influence of microsphere spacing on the superresolution was analyzed,and the imaging law was obtained.For the single microsphere scanning,the vertical optical tweezers system independently built by the research group was used to conduct imaging test experiments.It was determined that the method of capturing and controlling microspheres was feasible and the imaging was clear.Finally,the observation and scanning imaging of the sample by single microspheres under the system were realized.