| Background: Non-functional pituitary adenoma(NFPA)is a disease with a high incidence,which accounts for a large part of pituitary tumors and plays a pivotal role.While invasive NFPAs which have not any endocrinology manifestations and space-occupying symptoms at early stages account for about 30 percent of NFPAs.The purpose of the present academic work was to identify significant genes with invasive promotion and their underlying mechanisms.Methods: Gene expression profiles of GSE51618 was available from GEO database.There are 4 non-invasive NFPA tissues,3 invasive NFPA tissues and 3 normal tissues in the profile datasets.Differentially expressed genes(DEGs)between non-invasive NFPA tissues and invasive NFPA tissues were picked out by GEO2 R online tool.There were total of 226 up-regulated genes and 298 down-regulated genes.Next,we made use of the Database for Annotation,Visualization and Integrated Discovery(DAVID)to analyze Kyoto Encyclopedia of Gene and Genome(KEGG)pathway,gene ontology(GO)and Kaplan Meier Plotter.Then protein-protein interaction(PPI)of these DEGs was visualized by Cytoscape with Search Tool for the Retrieval of Interacting Genes(STRING).There were total of 141 up-regulated genes and 171 down-regulated genes.Of PPI network analyzed by Molecular Complex Detection(MCODE)plug-in,all 141up-regulated genes were selected.Results: After reanalysis of GO,five genes(ATP2B3,ADCYAP1R1,PTGER2,FSHβ,HTR4)were found to significantly enrich in the c AMP signaling pathway,Neuroactive ligand-receptor interaction and Renin secretion via reanalysis of DAVID.Conclusions: We have identified five significant DEGs with invasive promotion in invasive NFPAs on the basis of integrated bioinformatical methods,which could be potential therapeutic targets for invasive NFPAs patients. |
