Investigating The Expression And Clinical Significance Of C6orf89 In Human Hepatocellular Carcinoma Based On Bioinformatics

Objective: The expression and clinical significance of C6orf89 in human hepatocellular carcinoma(HCC)were evaluated based on multiple databases.Methods: The expression of C6orf89 in HCC was analyzed based on GTEx and TCGA databases and diagnostic ROC analysis,and the expression difference was verified by immunohistochemistry and Western Blot in clinical samples.UALCAN web tool was used to analyze the methylation level of C6orf89 in HCC,and Survival web server was used to evaluate the effect of C6orf89 methylation on the clinical prognosis of HCC.TIMER2.0 database was used to analyze the correlation between C6orf89 and tumor immune invasion.Metascape web tool is used for enrichment analysis and functional annotation analysis of C6orf89.Results: C6orf89 expression was higher in HCC tissues than in nontumour tissues(P<0.05);diagnostic ROC analysis resulted in an AUC=0.75,95% CI=0.80-0.69,suggesting that C6orf89 is a potential biomarker to distinguish HCC tissues from normal tissues;Compared with non-tumor tissues,C6orf89 showed hypomethylation in HCC(P < 0.01),and its methylation level was positively correlated with the prognosis of HCC patients;The expression level of C6orf89 was positively correlated with the infiltration of twelve kinds of immune cells,including mast cells(P=2.77e-09),cancer-associated fibroblasts(P=1.72e-05),CD8+T cells(P=8.5e-03),CD4+T cells(P=4.65e-02),etc.;enrichment analysis revealed that C6orf89-related genes involved in major bioregulatory pathways associated with epigenetic alterations and protein synthesis(P<0.01).Conclusion: C6orf89 gene is highly expressed in HCC tissues and is associated with methylation,immune infiltration,epigenetic changes and protein synthesis,which may be a potential target for the diagnosis and treatment of HCC.