Bioinformatics-based Analysis Of The Expression And Significance Of Small Ribonucleoprotein Polypeptide B2(SNRPB2) In Hepatocellular Carcinoma

Background and Objective:Liver cancer(HCC)is a very prevalent malignant tumor of the digestive system in China and around the world.Hepatocellular carcinoma(HCC)is the most common type of pathology in liver cancer,accounting for almost 90%of the total number of cases.Chronic hepatitis and liver cirrhosis are two of the major risk factors for hepatocellular carcinoma.Moreover,the delayed diagnosis caused by the late symptoms in hepatocellular carcinoma can affect the subsequent treatment outcomes.The discovery of new liver cancer markers is therefore an important direction for liver cancer research in order to improve diagnostic accuracy and therapeutic efficacy.The role of alternative splicing and splicing-related proteins in cancer has been studied more and more in recent years,and these factors have an important impact on many cancers.It is believed that alternative splicing is an important factor that leads to cancer progression.Therefore,any factors leading to aberrant mRNA splicing will affect cancer development.Small ribonucleoprotein polypeptide B2(SNRPB2)is an essential component of the U2 snRNP,and it plays a role in the maturation and splicing of mRNA.In this study,the effect of SNRPB2 on the proliferation and migration of hepatocellular carcinoma was investigated at three levels:tissue,cellular,and animal.Methods:1.Differences in SNRPB2 expression in hepatocellular carcinoma and paraneoplasia were analyzed by bioinformatics analysis of hepatocellular carcinoma data from TCGA and GEO,while the correlation between SNRPB2 expression and prognosis of hepatocellular carcinoma patients was explored using survival analysis and univariate multifactorial analysis.2.To confirm the difference in SNRPB2 expression between clinical liver malignant and normal tissues,three distinct techniques were used:Western blot,immunohistochemistry,and qRT-PCR.The cell lines employed in the study were selected using Western blot analysis of SNRPB2 expression levels in hepatocellular carcinoma cell lines.3.To check the effectiveness of siRNA and shRNA,Western blot and qRT-PCR were used.EdU and plate cloning assays were carried out to determine the effect of SNRPB2 expression on the proliferation ability of hepatocellular carcinoma cells.Nude mouse tumorigenesis assays were then used to determine the effect of SNRPB2 expression on the migration ability of hepatocellular carcinoma cells.4.Changes of key proteins in the PI3K/AKT/mTOR signaling pathway were investigated after changing the expression of SNRPB2 in liver cancer cells.Result:1.According to a public database analysis,hepatocellular carcinoma had significantly higher SNRPB2 expression than paraneoplasia,and this level of expression was also an independent prognostic factor for the disease.The significant high expression of SNRPB2 in hepatocellular carcinoma was once demonstrated by experimental validation of clinical liver cancer tissues.2.The proliferation and migration abilities of liver cancer cells HCC-LM3 and MHCC-97H were significantly reduced by inhibiting the expression of SNRPB2.3.The levels of p-PI3K,p-AKT,and p-mTOR protein expression were markedly decreased in HCC-LM3 and MHCC-97H when SNRPB2 expression was suppressed.Conclusion:1.SNRPB2 is highly expressed in hepatocellular carcinoma and is an influential factor in poor prognosis for HCC patients.2.By positively regulating the PI3K/AKT/mTOR signaling pathway,SNRPB2 promotes the proliferation and migration of liver cancer cells.