Characterization Of Glucose Detection Based On Microwave-assisted Synthesis Of "Nano-enzyme"

Diabetes has been paid more attention in recent years due to its prevalence and serious complications,blood glucose detection is particularly important in the prevention and treatment of diabetes.In the face of the increasing demand for real-time detection,it is urgent to develop simple,sensitive and stable micro glucose biosensor based on the construction of high-performance electrode materials.Therefore,the catalytic oxidation performance of zeolitic imidazolate frameworks on glucose was utilized in this paper,and"nano-enzyme"based on metal-organic framework materials was developed.Glucose was detected by electrochemical and photochemical methods,and integrated glucose sensor based on screen printing electrode was developed to explore the practical application value of the device.The main research contents of this paper are as follows:（1）ZIF67/ZIF8 composite electrode material was prepared by microwave-assisted synthesis method to achieve rapid and mass synthesis of"nano-enzyme",the glucose electrochemical detection characteristics of"nano-enzyme"were studied based on glassy carbon electrode.The rapid and simple synthesis of ZIF67/ZIF8 composites was investigated by microwave assisted synthesis method,and the morphology and structure of the composites were systematically characterized by electron microscopy and spectral analysis.ZIF67,ZIF8and ZIF67/ZIF8 were assembled on glassy carbon electrode,respectively.Based on the three-electrode system,the electrochemical response characteristics of ZIF67/ZIF8 to glucose were tested,and the mechanism of enzyme-free glucose detection was deeply analyzed.The ratio of each substance,electrode modification concentration,p H value of electrolyte and detection potential of the composite were adjusted to establish the optimal electrode detection conditions,and the standard curve of glucose detection concentration and current was established to obtain the sensitivity,detection lower limit,linear range and other performance indicators.The selectivity,stability and repeatability of the electrode were evaluated,and the response of the real human serum samples was analyzed by using the spiked recovery method to explore the practical application value of the electrode.（2）In order to further improve the sensitivity of glucose detection,a novel two-dimensional nano-material was introduced to develop ZIF67/Mo S₂/r GO composite electrode material and the photoelectric chemical glucose detection characteristics of"nano-enzyme"were studied based on glassy carbon electrode.ZIF67/Mo S₂/r GO composites were prepared by microwave-assisted one-step synthesis method.The structure and morphology of the composites were characterized by Scanning Electron Microscopy and X-ray diffraction.The band position of Mo S₂ was determined based on UV-vis and M-S spectra.ZIF67,ZIF67/Mo S₂ and ZIF67/Mo S₂/r GO were assembled on glassy carbon electrodes,respectively.Xenon lamp was used to provide light source,and the photoelectric response characteristics of ZIF67/Mo S₂/r GO to glucose were tested based on the three-electrode system.Energy band diagram was drawn to analyze the mechanism of photoelectric chemical glucose detection by electrode.The bias voltage,p H value of electrolyte and light intensity of electrode detection were adjusted to establish the optimal electrode detection environment,and the standard curve of glucose detection concentration and photocurrent was established to obtain the sensitivity,detection lower limit,linear range and other performance indicators.The selectivity,stability and repeatability of the electrode were explored,and the reliability of the actual detection of the electrode was evaluated through the detection of real human samples.（3）In order to improve the portability and immediacy of detection,a glucose sensor based on screen printing electrode was developed and the photoelectric chemical detection characteristics of"nano-enzyme"were studied.Conductive silver paste,conductive carbon paste,Ag/Ag Cl paste and insulating ink were printed layer by layer on PET flexible substrate to prepare an integrated electrochemical three-electrode system.ZIF67/Mo S₂/r GO composite material was modified on the working electrode to assemble a portable glucose biosensor.The photoelectric chemical detection platform was built to evaluate the intrinsic photoelectric chemical response characteristics of ZIF67/Mo S₂/r GO in the Screen-printing electrode system,and verify the feasibility of the device as a photoelectric chemical biosensor.The photoelectric chemical glucose detection characteristics of the device were explored by adjusting the detection parameters such as bias voltage,electrolyte p H value and test light intensity,and the standard curve of glucose detection concentration and photocurrent was established.By testing the photoelectric chemical response of the sensor to glucose in the electrolyte containing different interferers,the system evaluated its selectivity,and tested the stability and repeatability of the sensor,and applied it to the test of real serum samples.Thesis makes full use of zeolitic imidazolate frameworks material excellent electric catalytic activity,get rid of the use of enzymes and developed glucose biosensors based on"nano-enzymes",realized the sensitive and stable,accurate and reliable glucose detection,provides scientific basis and technical support for the development of wearable and other micro-intelligent biological detection devices.