Microwave Assisted Preparation Of Dendronized Magnetic Nanoparticles And Its Applications In Immobilization Of Biomacromolecules

Biomacromolecules such as proteins, enzymes, having been applied in clinical diagnosis, analysis and detection, drug delivery, receive widely attention. Biomacromolecules, as a typ of special molecules with biological activity, are susceptible to external environment such as temperature, pH and ionic concentration, resulting the destruction of the natural structure of biological molecules. Therefore, it is necessary to enhance the storage stability and the reusability of biological molecules by immobilization technology.In the current study, microwave-assisted synthetic technology was used to graft the PAMAM onto the surface of magnetic nanoparticles. The optimized conditions were investigated by single factor. TEM, XRD, FTIR, TGA, VSM and EA analyses demonstrated that the PAMAM dendrimer was successfully grafted onto the surface of the magnetic nanoparticles. The result indicated that third generation PAMAM modified magnetic nanoparticles could be obtained within36h using microwave irradiation, which is a fast and efficient method compared with the traditional heating methods that have been reported to require as many as18and30d.In this paper, the construction of the BSA-PMNPs was completed by grafting the BSA onto the surfaces of the PMNPs using glutaraldehyde as a crosslinker. UV-vis spectrophotometry, elemental analysis and CLSM were used to confirm the BSA binding capacities of the materials. Immunoaffinity approaches were used to investigate the activity of the BSA on the PMNPs. The direct separations of three different chiral amino acids with BSA-PMNPs were investigated to confirm the chiral recognition ability of the BSA-PMNPs. The separation results indicated that the BSA-PMNPs could be novel materials for chiral separation.A new type of immobilized HRP was prepared on polyamidoamine magnetic nanoparticles and confirmed by various characterization techniques. The enzymolysis conditions were investigated, including temperature, substrate concentration, time and pH. Four phenolic compounds were used as model substrates to investigate the catalytic mechanism of horseradish peroxidase on PMNPs. The results indicated that the transformation of toxic phenols into PPO plastic materials can be achieved through the catalysis of HRP.Microwave-assisted synthetic technology was applied in grafting PAMAM onto magnetic nanoparticles, which is a fast and efficient method compared with the traditional heating methods. The polyamidoamine magnetic nanoparticles can provide multiple attachment sites for the conjugation of various bioactive molecules and can also enhance the biocompatibility.