Differential Analysis Of Histone Modifications During Aging

OBJECETIVE : In recent years,the aging of our population has become more and more serious,bringing enormous pressure on families and society.Therefore,it is even more necessary to pay attention to the solution of health problems of the elderly in the context of the deepening population aging.With the development of high-throughput sequencing technology,massive data including epigenetic modifications have been obtained,and it has been found that abnormal alterations of epigenetic modifications have an important regulatory role in aging.Therefore,this study mainly explored the dynamic changes of four histone modifications,H3K4me1,H3K4me3,H3K27me3 and H3K27 ac,during the aging process and screened the target genes related to aging.METHODS: In this study,Ch IP-seq data of four histone modifications,H3K4me1,H3K4me3,H3K27me3 and H3K27 ac,and the corresponding RNA-seq data were obtained from public data.for the following analyses:(1)Dynamic analysis of the four histone modifications in different age groups and screening for key genes associated with aging;(2)Combined Ch IP-seq and RNA-seq analysis to screen for key genes associated with aging.To further explore the regulatory roles of the four modifications in aging,screen the potential target genes directly affected by the four histone modifications,and analyze the regulatory mechanisms of histone modifications on ageing-related genes.RESULTS:(1)Dynamic analysis of Ch IP-seq data revealed that the target genes in H3K4me1 with different enrichment intensities in young and old groups were SORL1,NFE2L2,PIK3R1 and SMAD6,and the enrichment intensities were young > old;the top 6 target genes in H3K4me3 modification had significant differences in enrichment intensities in young and old groups: young > old;only the promoters of ZC3H12 A and SRF in H3K27 ac modification had different enrichment intensities in different ages: young < old.Only the promoters of ZC3H12 A and SRF in the H3K27 ac modification had different enrichment intensities in different ages:young group < old group.(2)Combined Ch IP-seq and RNA-seq analysis revealed that only PIK3R1 expression level differed among the target genes of H3K4me1 differential site;2 genes differed among the target genes of H3K4me3 differential site(RHOB,NR4A3);only SRF expression level differed among the target genes of H3K27 ac differential site.CONCLUSION: Screening yielded histone modification target genes associated with aging.They were: H3K4me1(PIK3R1);H3K4me3(RHOB,NR4A3 and ARHGAP29);and H3K27ac(SRF),respectively.