| So far,there has been no special study on the phylogeny of Chinese Coptacrinae except for the species recorded in the description of new species and floristic studies.The taxonomic status of each genus of the Chinese Coptacrinae is still unclear,which needs further research and analysis.In this study,we obtained the mitochondrial genomes of 14 species through thenext generation sequencing,including 14 species belonging to 8 genera inCoptacrinae,and combined the relevant knowndata from NCBI to reconstruct the phylogeny for the purpose of testing the monophylyof Chinese Coptacrinae and the affiliation of each genus and species withinsubfamilies(or family).The main results are as follows:1.The mitochondrial genome characteristics of the 14 species of grasshoppers determined in this study were similar to those of the published mitochondrial genome of the locust family.The mitochondrial genomes of 14 species of locust have the lengths from 14829 bp to 18468 bp respectively.The A+T contents are all between 70%-80%,respectively,with the prominent A+T content.The gene rearrangements of all species are the same with the ancestral genearrangement of Caelifera,and including 22 tRNA genes,2 rRNA genes,13 PCGsand 1 A+T rich region(between rrnS and trnI).The gene distribution characteristics,the number and length of gene interspaces and overlapping regions,and the secondarystructure of 22 tRNAs are mainly G-U mismatch,which are consistent with other speciesof Caelifera.Among the 13 protein-coding genes of 14 grasshoppers,there are 9 speciess had non-standard start codons:the start codon of ND2 in Apalacris varicornis,Apalacris dupanglingensis,Apalacris tonkinensis,Eucoptacra kwangtungensis,Eucoptacra praemorsa,Eucoptacra binghami,Coptacra yunnanensis,Epistaurus aberrans was GTG,and all other than that protein-coding genes were typical ATN start codons.TAG as a start codon,appears many times in each species and is more common.The termination codon of ND1 in all species is TAG,most of the termination codons are typical TAA forms,and some species have incomplete start codons TA and T.2.The Chinese Coptacrinae is not a monophyletic group.Based on the mitochondrial genome of 13 protein-coding genes from 112 samples and 4 ribosomalgene sequences from 87 samples,Filchnerella kukunoris and Filchnerella yongdengensis were selected as outgroup,and the phylogenetic tree was constructed by maximum likelihood method(ML)and Bayesian method(BI).The BI tree constructed by the 4 ribosomal genes showed similar results to the ML tree constructed by the mitochondrial genome PCGs.Whether it is the mitochondrial genome or the phylogenetic tree constructed by ribosomal genes,the subfamily Coptacrinaeis not monophyleticgroups,but the 3 species(Eucoptacra,Apalacris,Coptacra)of the typical group Coptacriniare clustered together.The genus Traulia form separate branches,which are always close to but not merged with the above branches.Assamacris also formed a separate branch,and it was close to the Pseudotraulia.In this study,the mitochondrial genomes of 14 grasshopper species in Coptacrina are firstly sequenced and analyzed.The systematic classification was discussed from the perspective of molecular biology,which provided the molecular basis to confirm the taxonomic status of Chinese Coptacrinae species. |
