Effects Of Heavy Metal Cadmium On The Important Genes Of Sex Pheromone Biosynthesis Pathway In The Asian Corn Borer,Ostrinia Furnacalis

The Asian corn borer(Ostrinia furnacalis),as one of the main pests on corn in China,causes serious damage and economic losses to corn.As an important communication factor for moth insect population reproduction,sex pheromone plays an important role in green prevention and control.Previous studies have found that the effective components of its sex pheromone decreased after Asian corn borer was stressed by cadmium.In order to gain an in-depth understanding of the mechanism of sex pheromone regulation of Asian corn borer under heavy metal cadmium stress,this paper carried out transcriptome sequencing,cloning and relative expression determination of important genes,and dehydrogenase dehydrogenase of adult Asian corn borer under cadmium stress.Transcriptome analysis showed that there were differences in the expression of some genes in metabolic pathways before and after cadmium stress,among which the fatty acid metabolism pathway was closely related to the sex pheromone biosynthesis pathway.In this paper,PCR technology was used to obtain the Ostfur PBAN gene sequence,603 bp long.The translation precursors undergo cleavage processing to form five neuropeptides,DH-PBAN,α-SGNP,β-SGNP,PBAN and γ-SGNP,which are completely consistent with the amino acid sequence of European corn borer.In order to explore the expression of pathway-influencing factors under cadmium stress,the expression of PBAN,PBANR andΔ14-desaturases genes in different parts of Asian corn borer was detected in this paper.PBAN gene was significantly down-regulated in the head which is its main expression site.PBANR was significantly down-regulated in the abdomen and showed a decreasing trend.The expression of Δ14-desaturases was only decreased in the head.The results showed that cadmium had a significant effect on enzyme activity in addition to gene expression.In this paper,the activities of Δ14-desaturases in the head,chest and abdomen were also detected after that female adults were stressed by different concentrations of Cd.The effect mechanism of Cd stress on the synthesis of O.furnacalis sex pheromone in vivo shows different differences from upstream regulators to downstream intracellular pathways,from gene expression to activity,and a more specific mechanism needs to be Further research.