Research On Spatial Light Interference Microscopy Based On Holoeye Pluto SLM

Quantitative phase imaging(QPI)is a kind of micro interference technology which has been well developed in recent years.It has the characteristics of non-invasive,fast and high resolution.Traditional optical imaging records the change of amplitude,but many evidences show that the phase of light contains more object information than the amplitude,and is more sensitive to the structure of the object.In 2011,Professor G.Popescu invented Spatial light interference microscopy(SLIM)based on Zernike phase contrast microscope.SLIM uses incoherent light illumination to overcome the influence of speckle noise,and uses common path interference to make the system have high inherent phase sensitivity.Spatial light modulator(SLM)can dynamically modulate the phase.By introducing different phase shifts into SLM,the phase to be measured is solved by four step method.The phase is unwrapped by the improved quality-guided path following,and the reconstructed microsphere image is obtained.In this study,SLIM and Holoeye pluto SLM were used for quantitative phase imaging of microspheres,and an improved quality-guided path following was proposed to reconstruct the three-dimensional profile of microspheres.It is found that temperature has an effect on the phase of liquid crystal spatial light modulator,and the influence of temperature and modulation phase is studied.The whole system realizes the microsphere reconstruction,analyzes the noise problem,and improves the imaging quality.The improved quality-guided path following reduces the error and improves the accuracy of understanding the package.