Design Of Single-cell Electrorotation Microfluidic Chip Based On Digital Circuit Control

All substances possess some degree of dielectric properties.Under the action of electric field,the material itself can polarizable to a certain extent,and the internal charges of the material will be arranged in order along the direction of the applied electric field after polarization.If the applied electric field is uneven,the polarized particles will be moved by the dielectrophoretic force.Using electric field to control the electrophoresis of particles,the electrophoresis force can be controlled by changing the parameters of electrical signals.Combined with optical,acoustic,electromagnetism and other technologies,the control and analysis of particles can be realized on microchips.Dielectrophoresis microfluidic chips have been widely used in various biological control fields because of their many advantages,such as low production cost,convenient operation and high throughput.Dielectrophoresis has played an important role in many fields of single cell research,such as measurement of single cell electrical parameters,single cell imaging,etc.However,most of the current dielectrophoresis electro-rotation chips are based on a number of two-dimensional planar electrode structures.In practical experiments,such planar chips often have some limitations,such as small effective working distance,weak dielectrophoresis force,fast attenuation of electric field,uneven distribution of electric field intensity and other shortcomings.In order to solve the limitations of the electrodes in the two-dimensional plane,we reconstructed a multi-electrode structure and realized the rotation of electric field through the control method of digital circuit,thus solving the difficulty of applying multi-electrode synchronous signals.At the same time,compared with the three-dimensional structure of the electrode dielectrophoresis chip,the difference between the two-dimensional plane electrode and the three-dimensional vertical electrode was compared and analyzed in simulation,and the feasibility of the three-dimensional vertical electrode structure was verified.In this paper,the electrode structure was analyzed by simulation optimization method,and the differences between the two electrode structures were compared and analyzed.Optimized design was carried out on the multi-electrode electro-rotation structure to realize the functions of single cell capture,spatial positioning and three-dimensional rotation,which expanded the functions of the multi-electrode structure.