Study On Rare Earth Fluorescence Temperature Microscopic Imaging Technology

Temperature is an important parameter in scientific research and industrial production.In the field of biomedicine,accurate temperature measurement is closely related to the treatment of diseases,while in industrial production,temperature distribution information affects the normal operation of industrial production.When measuring temperature in these areas,it is not only required that the temperature measurement accuracy is not interfered by external factors,but also that the entire temperature field of the measured object can be obtained.However,the existing contact and non-contact temperature measurement technologies have their own limitations and cannot meet these stringent temperature measurement requirements.Therefore,this article proposes a temperature field temperature measurement method based on the combination of fluorescence intensity ratio and digital image processing.This method can quickly,real-time,and accurately measure the target temperature field continuously,and both the temperature measurement efficiency and the temperature measurement accuracy are obtained.A substantial improvement.Since the biomedical field has the most stringent requirements for temperature sensors,when selecting temperature sensors in this article,the high-temperature sensitive rare earth up-conversion material Na YF₄:18%Yb³⁺,2%Er³⁺@Na YF₄ is used as temperature-sensitive probes to meet future applications Long-term goals in the field of biomedicine.This paper develops a micro-scale temperature imaging system based on the fluorescence intensity ratio(FIR)of the two emission bands of 520nm and 540nm of temperature-sensitive materials.The spatial distribution intensity information of the two spectral bands is recorded by the camera after passing through two different narrow-band filters,and finally the collected image information is processed by the computer to obtain the temperature distribution map of the target,thereby realizing temperature imaging at low cost.In this study,the FIR temperature measurement method of rare earth up-conversion materials is theoretically derived,and the hardware design of the temperature imaging system is carried out according to the relevant theoretical basis.Then,digital image processing technology is used to perform denoising,filtering and image segmentation processing on the obtained effective gray image.Finally,the temperature is calculated according to the R-T relationship obtained by the temperature calibration,and the pseudo-color transformation is performed on the obtained temperature map to obtain the temperature distribution map.In order to verify the feasibility of the system,the article first conducts temperature imaging experiments on temperature-sensitive materials.The experimental results show that the expected experimental goals can be achieved when the temperature-sensitive materials cover a large area.In order to expand the application field,temperature imaging experiments were added to the cells and heating wires.The experimental results show that the research method used in this article is technically feasible,but the accuracy of temperature measurement needs to be improved,and a lot of research is needed in the follow-up.