Physiological Index And Proteomic Study Of Alfalfa Mycorrhizal Symbionts Under Atrazine Stress

Mycorrhiza is a symbiosis between plants and fungi,which is very beneficial to either one or both.Up to now,more than 80% of the roots of terrestrial plants have been able to coexist with glomus fungi in the soil,The most common mycorrhizal fungi are AM(Arbuscular Mycorrhizal)fungi,is abled to establish symbiotic relationships with most plants.In the symbiotic state,AMF gives plants a lot of good properties,such as the ability to detoxify heavy metals,degrade pesticides,repair saline and alkaline soils,resist plant diseases and insect pests,and so on.At present,the research on AM fungi repair damaged ecosystem has become the focus of research at home and abroad.This study selected the dominant strains for the Mose tube handle(Funneliformis mosseae),fungus symbiotic crop alfalfa(Medicago sativa),the previous experimental results have shown that mycorrhizal alfalfa is the best crop research mechanism of atrazine degradation.This study based on the previous experimental results,cultivation of mycorrhizal material by Hoagland nutrition on Physiological Indexes of symbiotic system were determined,and with the help of protein iTRAQ in roots of plant technology on atrazine stress symbiosis of Alfalfa under mycorrhizal analyzed expression changes,further reveal the physiological and molecular mechanisms of degradation of atrazine in mycorrhizal plants.The spatial expansion index,material accumulation index,photosynthetic ability,stress resistance and antioxidant defense ability of mycorrhizal alfalfa were improved compared with that of non mycorrhizal alfalfa.Specific performance in mycorrhizal alfalfa in tolerate atrazine stress within sixth days after the stem length,diameter and root shoot ratio compared to non mycorrhizal alfalfa were increased by 31.31%,54.79% and 75%;the root,stem and leaf dry weight than the non mycorrhizal alfalfa compared were increased by 119.20%,29.04% and 26.45%;the chlorophyll a,chlorophyll B and total chlorophyll content than non mycorrhizal alfalfa compared were increased by 21.06%,44.30% and 10.87%;the net photosynthetic rate Pn transpiration rate Tr and stomatal conductance Gs than non mycorrhizal alfalfa compared were increased by 80.77%,550% and 191.18%;the leaf relative conductivity than non mycorrhizal fungi alfalfa decreased by 16.75%;the root MDA content than non mycorrhizal alfalfa was reduced by 11.03%;the proline content in roots than in non mycorrhizal alfalfa increased by 53.96% compared to its root TTC reductive intensity;The PPO,POD,CAT and SOD activity of roots were increased by 26.31%,37.51%,41.20%,and 70.29%,respectively,compared with that of non mycorrhizal alfalfa.Selected changes,atrazine stress of mycorrhizal root protein of Alfalfa by iTRAQ technology showed that significant differences were identified for protein 533,which up-regulated protein 276,down 257.For further analysis of atrazine stress AMF and molecular mechanism of organism to improve alfalfa symbiosis,the protein information,GO classification information,GO Level database on 2 classified information,KEGG classification information and related references,will have different functional annotation of proteins related to protein degradation of atrazine,atrazine stress response related proteins involved in plant immunity response related protein,protein synthesis,protein translation,signal processing,transfer and biological processes related to protein classification.