Study On Predictive Model Of Serum Irisin Concentration After Fitness With Different Exercise Programs

Objective:Muscle factor Irisin can act on a variety of tissues or organs of the body and play a beneficial role.The purpose of this study is to explore the potential factors affecting the changes of serum Irisin concentration in Chinese Han sedentary people and build a prediction model,so as to provide a reference basis for formulating a personalized fitness guidance plan to maximum increase of serum Irisin concentration.Methods:411 ordinary Han Chinese（male:181,female:230;age:41±15 years old;height:165.77±8.13cm;BMI:23.34±3.10kg/m~2）aged 20-69 with no regular physical exercise habits in the past year,who completed 16-week exercise intervention with complete basic index（sex,age,BMI）,glucose and lipid metabolism index（TG,HDL-C,LDL-C,CHOL,GLU）,cardiopulmonary function index（VO₂max）and DNA extraction were selected from the sample database established by"Key Technology Research of Scientific Fitness Expert Guidance System and Service Platform（2012BAKB23）",supported by the 12th Five-Year Plan of the Ministry of Science and Technology.The exercise methods include mountaineering,running on the playground,power cycling and comprehensive intervention of aerobic plus strength.Serum Irisin concentration was determined by enzyme-linked immunosorbent assay before and after exercise intervention.Irisin-related gene FNDC5 was selected as the candidate gene.Single nucleotide polymorphism site screening was performed by Haploview and reference.Genotyping was performed by time-flight mass spectrometry.The indexes affecting the concentration of serum Irisin in the references were divided into four levels:the first index（basic index）,the second index（glucose and lipid metabolism index）,the third index（cardiopulmonary function index）and the fourth index（FNDC5polymorphism index）.The data were analyzed by PLINK1.9 and SPSS21.0 software,individual Cohen’s d effect（ES）was calculated,ES<0.2 is invalid,ES≥0.2 is valid.The effects of four levels of indicators on serum Irisin concentration were analyzed by covariance and gradually incorporated into multiple linear stepwise regression（initial value）and logical regression（training effect）to build a prediction model.Besides,the function of positive SNP was interpreted based on the differences of FNDC5 gene expression data of different genotypes of carriers published by GETx database.Results:1)Initial value:The serum Irisin concentration of sedentary people varied from2.51 to 79.82 ng/ml,which was affected by sex,age,obesity,VO₂max,blood lipid and genetic factors.（1）Male:Lg（Irisin）=1.265+0.006*VO₂max,R~2=0.581.（2）Female:model 1:Lg（Irisin）=1.117+0.150*HDL-C,R~2=0.347,model 2:Lg（Irisin）=1.202+0.141*HDL-C-0.049*rs3480,R~2=0.365.The rs3480 polymorphism of female FNDC5was significantly associated with serum Irisin concentration,GG2max is an effective predictor of serum Irisin concentration in men.HDL-C and FNDC5 rs3480 loci are effective predictors of serum Irisin concentration in women.2)Different exercise programs have different effects on serum Irisin concentration in men and women.Different exercise programs have different effects on the concentration of serum Irisin.Comprehensive intervention of aerobic plus strength is an effective exercise mode to increase the concentration of serum Irisin in sedentary men.3)There are great individual differences in the training effect of serum Irisin concentration.Exercise program,FNDC5 rs16835198 and rs3480 are the predictors of the training effect of male serum Irisin concentration.HDL is the predictor of the training effect of sedentary female.These should be used as important reference factors when formulating a personalized fitness guidance plan to maximum increase of serum Irisin concentration.