Analysis Of Epidermal Growth Factor Receptor Family In Gastric Cancer Induced By HP Infection

ObjectiveThe objective of this study was to investigate the difference between helicobacter pylori（HP）-induced gastric cancer and non-HP-induced gastric cancer.The expression of epidermal growth factor receptor family（ERBB receptor family）was used as the main line.In this study,the clinical detection methods of HP,the clinical characteristics of gastric cancer and the expression of ERBB molecules were studied.First,a precise and reliable grouping method was sought to identify HP infection,then the differences among the clinical macro features were analyzed,and then the expression of ERBB family related to gastric cancer was verified and analyzed by classical experimental methods.Finally,the author tries to find the differences between gastric cancer with different etiology,so as to provide clues for the development of individualized and precise treatment plans and the search for molecular targeted drug resistance related factors,so as to achieve the purpose of precise treatment.MethodGastric cancer tissue samples were collected from the General Surgery ward of Lanzhou University Second Hospital from February 2021 to December 2021.Gastric cancer patients were grouped by C13 breath test,tissue rapid urease and PCR,and gastric cancer tissues were divided into HP positive and negative groups.Clinical data were collected and tumor stages and pathological manifestations were analyzed.Western Blot,ELISA and IHC were used to verify the expression of EGFR,Her2,Her3 and Her4 in cancer and normal tissues of HP negative and HP positive groups.The experimental data were expressed as mean ± standard deviation（`x±SD）.Paired sample T test was used to compare the data of the two groups,and the measurement data of multiple groups were analyzed by single factor analysis.SPSS 20.0 software was used for comparative analysis of the data,and it was concluded that P<0.05 was considered statistically significant.ResultsThere were statistically significant differences in EGFR,Her3 and Her4 expression in the ERBB family between CagA-positive and CagA-negative gastric cancers infected with HP,while there was no difference in Her2 expression.1.EGFR: In patients with gastric cancer in the CagA positive group and the CagA negative group,the expression of EGFR in cancer tissues was different from that in normal tissues.In the CagA negative group,the expression of EGFR in cancer tissues was higher than in normal tissues,while in the CagA positive group,the expression of EGFR in normal tissues was higher than in cancer tissues（P<0.05）,statistically significant.EGFR is mainly distributed around gland ducts and muscle layer,and is expressed in cell membrane and cytoplasm.2.Her2: There was no difference in Her2 expression between CagA positive gastric cancer and CagA negative gastric cancer patients（P>0.05）,no statistical significance.3.Her3: There were differences in the expression of Her3 in cancer tissues and normal tissues between CagA positive and negative gastric cancer patients.The expression of Her3 in cancer tissues was higher than that in normal tissues in both groups.Her3 was mainly distributed around the mucosal layer and scattered in the muscle layer,and the expression of Her3 was found in the cell membrane and cytoplasm.（P<0.05）,statistically significant.4.Her4: There were differences in the expression of Her3 in cancer tissues and normal tissues between CagA positive and negative gastric cancer patients.The expression of Her4 in cancer tissues was higher than that in normal tissues in both groups.（P<0.05）,statistically significant.Conclusions:1.EGFR expression was higher in normal tissues than in cancer tissues in CagA positive gastric cancer patients.The expression of EGFR in normal tissues was lower than that in cancer tissues in CagA-negative gastric cancer patients.2.The expression of Her2 in tissue specimens of CagA positive and negative gastric cancer patients was not statistically significant.3.High expression of Her3 and Her4 was found in tissue samples of CagA positive and negative gastric cancer patients.