The Effect Of NLRC5 On PD-L1 In Endometrial Carcinoma

Aims: We aimed to explore whether NLRC5 could regulate the programmed cell death protein ligand 1（PD-L1）in EC.We also investigated the related molecular which led to inactivation of NLRC5 in EC.Methods: The expressions of NLRC5 and PD-L1 in endometrium tissue microarray were detected by immunohistochemistry.Pearson correlation analyse was performed to detect the expression correlation between NLRC5 and PD-L1.Immunofluorescence staining,western blotting,and quantitative real time PCR（q RT-PCR）were used to detect the role of NLRC5 in PD-L1 in EC cell lines.The somatic mutation in EC patients was detected by whole-exome sequencing（WES）.Results: NLRC5 was down-regulated in the endometrium of EC patients when compared to those in the normal endometrium.The level of PD-L1 in the endometrium of EC patients was higher when compared to those in the normal endometrium.There was a negative expression correlation between NLRC5 and PD-L1.NLRC5 could promote the expression of PD-L1 in EC cell lines.The mutations of ANKRD20A2,C2orf42,ADGRB3,AVPR2,GOLGA6 C,IPPK may lead to down-regulation of NLRC5 in EC patients.Conclusion: NLRC5 could inhibit the activation of PD-L1 in EC.Mutations of ANKRD20A2,C2orf42,ADGRB3,AVPR2,GOLGA6 C,IPPK may lead to down-regulation of NLRC5 in EC patients.