Cervicovaginal Microenvironment Analysis Associated With Cervical Cancer Via 16S RDNA Sequencing And Metabolomics

Objective:A growing number of studies have shown that microecology is related to cancer,and the correlation between cervicovaginal microecology and cervical cancer is still unclear.This study aims to explore the relationship between cervicovaginal microecology and cervical cancer for discovering potential biomarkers.Methods:We performed 16S rDNA sequencing and metabolomic analysis in the cervicovaginal fluid from 10 LSIL patients,10 HSIL patients,10 CC patients and 10 healthy controls to reveal the differential florae and metabolites during cervical carcinogenesis.Results:Carcinogenesis was associated with alterations in microbiome diversity,individual taxa,and functions with notable changes in Lactobacillus,Prevotella and Aquabacterium,as well as in cervicovaginal metabolites that correlated with cervicovaginal microbial patterns.Lipids were significantly declined in LSIL and HSIL compared to HC,and significantly elevated in CC compared to LSIL and HSIL.Pathway enrichment analysis found phenylalanine,tyrosine and tryptophan biosynthesis pathway was crucial in cervical carcinogenesis.Conclusion:These results showed that microbic and metabolomic profiling are capable of distinguishing CC from precancer and highlighted potential biomarkers for the early detection of cervical dysplasia.It is a non-invasive approach which may allow the development of new strategies for the management of women at high-risk for cervical cancer.