Study On The Improvement Of The Quality Standard Of Sangjiang Ganmao Injection And The New Way Of Administration

Sangjiang Ganmao Injection is a pure Chinese medicine preparation produced by Shanxi Canonda Huayu Pharmaceutical Co.,Ltd.The formula contains six medicinal materials: mulberry leaf,chrysanthemum,perilla,forsythia,bitter almond and dried ginger.The six herbs are extracted by water.Prepared after extraction and separation by sedimentation.Sangjiang Ganmao Injection has the effects of dispelling wind,clearing heat,eliminating phlegm and relieving cough,and is mainly used to treat colds,coughs,headaches and sore throat.The clinical medication method is intramuscular injection,which has a relatively significant effect on wind-cold and wind-heat type colds.Currently,there are many traditional Chinese medicines on the market for the treatment of colds,and Sangjiang Ganmao Injection is safe,effective and reasonable in price.It is a variety worthy of promotion.However,there are very few studies on Sangjiang Ganmao Injection.The chemical composition of the medicine and its effects The mechanism of action,related targets and pathways of the treatment are unknown.The "Ministry-Promulgated Drug Standards" drafted by the Shanxi Provincial Institute for Drug Control on the quality control of Sangjiang Ganmao Injection is also relatively rough and needs to be improved.In order to make Sangjiang Ganmao Injection a more targeted and effective product,it is necessary to complete,supplement and refine its quality research,and to carry out the secondary development of Sangjiang Ganmao Injection.Objective1.Systematic identification of the chemical composition properties of Sangjiang Ganmao Injection,study its material basis,and provide a basis for effective control of the quality of Sangjiang Ganmao Injection.2.Establish TLC and content determination methods for Sangjiang Ganmao Injection,as well as the fingerprint of the finished injection,to provide a new quality control standard for Sangjiang Ganmao Injection.3.Compare the therapeutic effects of different administration routes of Sangjiang Ganmao Injection explore the possibility of multiple routes of administration,and provide reference for the best way of administration for clinical treatment of children’s colds.4.Analyze the mechanism of action of Sangjiang Ganmao Injection,and predict its possible therapeutic targets and pathways.Methods1.UPLC-Q-Orbitrap HRMS technology is used to quickly identify the chemical components contained in Sangjiang Ganmao Injection.2.A TLC method was established for Sangjiang Ganmao Injection with a reference substance of forsythin as the control.The content of forsythin and chlorogenic acid in many batches of Sangjiang Ganmao Injection was determined,and the fingerprint of the finished injection was established.3.Use animal models to investigate the therapeutic effects of Sangjiang Ganmao Injection on symptoms such as fever,inflammation,relieving cough and relieving asthma after being administered by different routes of administration.4.Based on UPLC-Q-Orbitrap HRMS results,use network pharmacology technology to predict targets and pathways.Result1.Identified 54 chemical components in Sangjiang Ganmao Injection,including25 flavonoids,10 phenolic acids,5 volatile oils,4 coumarins,and 10 other ingredients.2.The quality standards of Sangjiang Ganmao Injection have been improved by establishing stable and reliable TLC identification of forsythin reference substance,determination of chlorogenic acid and forsythin content,and fingerprint of injection finished product.3.By comparing the efficacy of Sangjiang Ganmao Injection in antipyretic,antiinflammatory,anti-tussive,and anti-asthmatic aspects through different administration routes,it is comprehensively considered that the more appropriate mode of administration is rectal administration.It provides a basis for the possibility of the drug in the clinical treatment of children with colds.4.Sangjiang Ganmao Injection may interfere with the expression of inflammatory cytokines by acting on targets and pathways associated with inflammation and the immune system,and regulate the body’s immune function as a whole,thereby exerting a therapeutic effect.ConclusionIt can be seen from the above results that this subject has been re-developed based on the existing Sangjiang Ganmao Injection,clarified its chemical substance basis,improved the quality standard of the drug,and explored the pharmacological effects and treatment of the drug The mechanism provides a basis for the quality standard control of Sangjiang Ganmao Injection and lays a foundation for later clinical application research.