Study On Quality Standard Of Qianglinaoqingsu Tablet Based On The Concept Of Quality Marker Of Traditional Chinese Medicine

Objective:To preliminarily predict the quality markers of Qianglinaoqingsu tablets and establish the quality evaluation method based on the quality markers.Methods:(1)Using UPLC-MS technology,Syncronis(2.1×100 mm,1.7 μm)C18column was used.The mobile phase consisted of water-acetonitrile and gradient elution.The flow rate was 0.2ml /min.The column temperature was 30 ℃.The injection volume was 2L.The ion source is electrospray ion source(+ ESI).Spray voltage 3.5k V;Evaporation temperature 320 ℃;Desolvation temperature: 320 ℃;Sheath flow 30 arb;Auxiliary airflow 8 ARB;The resolution of 70,000(FWHM)was used to quickly analyze the chemical constituents of the Qianglinaoqingsu tablets.(2)The identified compounds were screened by network pharmacology method,and the chemical components identified by mass spectrometry were predicted by BAT-Man-TCM platform.Based on the STRING database,the key targets in the fluttering parameters selection network for protein interaction analysis of potential drug component targets and disease targets,as well as the direct action targets of chemical components with high scores,were verified by molecular docking with the selected active components.(3)On the premise that the key components were obtained by UPLC-MS and network pharmacology,the intermediates in the prescription were qualitatively analyzed by TLC.(4)A DAD-HPLC method was established for the quantitative analysis of Qianglinaoqingsu tablets.Water and acetonitrile were used as mobile phase,gradient elution procedure was used,column temperature was controlled at 30℃,and detection wavelength was 220 nm.(5)HPLC fingerprint of Qianglinaoqingsu Tablets was established and analyzed by combining similarity evaluation and stoichiometry.Results :(1)The compounds obtained by UPLC-MS combined with literature included syriloside,isosinidine,schisandrol A,protocatechuic acid,etc.,which laid a foundation for the quality markers of Qianglinaoqingsu Tablets.(2)Ten active ingredients were obtained by screening the identified compounds through network pharmacology,and six active ingredients were further obtained by molecular docking based on the key targets in the network.(3)The thin layer identification of the intermediates in Qianglinaoqingsu tablet has good specificity and durability.(4)The linear ranges of syringoside,acanthopaniloside E and isofraxidinwere 49～735μg/ m L(r=1),39.7～596μg/ m L(r=0.9991)and 9.2～142μg/ m L(r=0.9994),respectively.The average recoveries were 95.48%,98.22%and 100.75%,and the contents of syringoside,acanthopaniloside E and isofraxidin were3.34,1.85 and 0.74 mg/g,respectively.(5)HPLC fingerprint results showed that there were 11 common peaks in Qianglinaoqingsu tablet,and 6 common peaks were identified,which were syringoside,isofraxidin and acanthopanax E in acanthopanax extract.Schisandrol,Schisandrol A and Schisandrol B in Schisandrol Extract.The similarity of 15 batches of qiangnaocinin tablets was greater than 0.95,which indicated that the similarity was good.15 batches of qianglinaoqingsu tablets were clustered into 5 classes.Principal components syringoside,isofraxidin,acanthopanax E;Schisandrol,Schisandrol A and schisandrin B in Schisandrol Extract were the main factors affecting the quality evaluation of Qianglinaoqingsu Tablets.Conclusion : This study integrated chemical efficacy composition formula compatibility,realize the Qianglinaoqingsu Tablets element film quality markers found that quality markers for traditional Chinese medicine research provides a feasible way of thinking,the establishment of the quality evaluation method based on the quality of markers for scientific and feasible powerful brain clear quality standard finally laid a good foundation.