The Expression And Clinic Implications Of Esophageal Cancer Related Gene 4 In Colon Cancer

Aim: Esophageal cancer related gene 4(ECRG4)is a key cell cycle protein.Previous studies demonstrated that ECRG4 play a critical role in multiple cancers development and progression,including esophageal cancer,lung cancer,breast cancer and gastric cancer.Moreover,the expression of ECRG4 in those cancers is significantly correlated with the prognosis,indicating ECRG4 is an important cancer mediator.However,whether ECRG4 also involved in colorectal cancer initiation and development still unknown.Methods: Colorectal cancer tissues were collected from patients undergoing operative treatment.Western blot,Real-time PCR and immunohistochemistry(IHC)were used for detecting the location and expression levels in cancer tissues.The additional colorectal cancer data derived from the Cancer Genome Atlas(TCGA)database was used to analyses the expression of ECRG4 in normal and colorectal cancer tissues and the prognosis between ECRG4 levels and colorectal cancer.The colorectal cancer cell line HCT-116 was used for in vitro experiment.Small interfering RNA(siRNA)was used for silencing ECRG4 in HCT-116 cells.After 48 hours transfection,Western blot and Real-time PCR were used for detecting ECRG4 protein and mRNA expression.The proliferation of siRNA transfected HCT-116 cells was measured using FITC-labeled Edu kit.The protein expression level of cleaved caspase 3 was detected by Western blot to measure apoptosis.The migration and invasion ability of siRNA transfected HCT-116 cells were measured by Transwell assay.Results: 1)The ECRG4 was predominant expressed in cytoplasm.Moreover,compared with normal colorectal and adjacent colorectal cancer tissues,the protein and mRNA levels of ECRG4 were significantly decreased in colorectal cancer tissues.2)The expression of ECRG4 in colorectal cancer tissues was negative associated with prognosis.3)siRNA transfection significantly decreased protein and mRNA expression in HCT-116 cells.4)Silencing ECRG4 promoted HCT-116 cells proliferation.5)Silencing ECRG4 inhibited HCT-116 cells apoptosis.6)Silencing ECRG4 promoted HCT-116 cells migration and invasion.Conclusion: 1)The expression of level of ECRG4 is a potential biomarker for assessment of colorectal cancer prognosis.2)The decreased expression of ECRG4 may promote colorectal cancer development and progression through promoting colorectal cancer cells proliferation,migration and invasion and inhibiting cell apoptosis.