The Expression Of KIF18B In Ovarian Carcinoma And The Correlation With Clinical Significance

Background:Ovarian cancer（OC）is a common gynecological malignancy.Because of its insidious onset,about 70%of patients are diagnosed at an advanced stage and have a high mortality rate among gynecological tumors.The American Cancer Center predicts that there will be 21,470 new cases and 13,770 deaths in 2021,and its mortality rate ranks fifth in female malignant tumors.Although the mode of appropriate opportunity of tumor cytoreductive surgery and postoperative platinum-based chemotherapy ± targeted therapy has become increasingly mature and standardized,the prognosis of ovarian cancer patients is still unsatisfactory.Tumor genome mapping data shows that there are complex and diverse genetic alterations in ovarian cancer,including gene mutations,deletions,and amplifications.Microtubule-kinesin family member 18B（KIF18B）plays an important role in the dynamic changes of microtubules and intracellular substance transport.It is a motor protein and a variety of cancer suppressor genes discovered in recent years.The up-regulation of KIF18B inverts the abnormal proliferation and malignant transformation of cells.In this study,we revealed the expression of KIF18B in ovarian cancer and its correlation with clinicopathological features and prognosis.Methods:1.GEPIA database,CSIOVDB database and K-M Plotter website were used to investigate the expression of KIF18B in epithelial ovarian cancer,and its relationship with histological type,pathological grade and FIGO stage,furthermore,to analyze its prognostic value.2.The expression of KIF18B in epithelial ovarian cancer patients and normal patients was analyzed retrospectively by immunohistochemical method.3.To analyze the correlation between KIF18B and clinicopathological features and prognosis of epithelial ovarian cancer and further verify the reliability of the results of the previous credit analysis.4.To investigate the expression status of KIF18B in ovarian cancer at the tissue level and cell line level by Western blot and RT-PCR validation.Results:1.The results of bioinformatics analysis showed that KIF18B was significantly highly expressed in the tissues of patients with epithelial ovarian cancer,and its expression was correlated with the characteristics of patients such as FIGO stage,histopathological type,and pathological grade,and the prognosis of patients with high KIF18B expression was poor.2.The expression levels of KIF18B in 56 cases of epithelial ovarian cancer and 35 cases of normal ovary and fallopian tube were detected by immunohistochemical staining.The results showed that KIF18B was mainly localized in the cytoplasm and cell membrane in epithelial ovarian cancer.Compared with normal tissues,KIF18B was significantly highly expressed in epithelial ovarian cancer tissues.In addition,high KIF18B expression was correlated with FIGO stage,pathological grade,positive lymph node metastasis,histological type and prognosis,but not with patient age,pretreatment serum Ca125 level,ascites and other factors,which well verified and supplemented the previous credit analysis.3.Western blot and RT-PCR were used to detect the expression of KIF18B in ovarian cancer tumor tissues and normal ovarian and fallopian tube tissues.The results showed that the expression of KIF18B in tumor tissues was higher than that in normal tissues.4.The expression of KIF18B in normal ovarian epithelial cell lines and ovarian cancer cell lines was detected by Western blot.The results showed that the expression of KIF18B in ovarian cancer cell lines was higher than that in normal ovarian epithelial cell lines.Conclusions:1.It is demonstrated that KIF18B is highly expressed in epithelial ovarian cancer,suggesting that the elevated expression of KIF18B may be associated with the development of epithelial ovarian cancer.2.The expression of KIF18B in epithelial ovarian cancer is correlated with clinicopathologic features such as clinicopathologic stage,histopathological type,and lymphatic metastasis.3.The high expression of KIF18B has a correlation with poor prognosis in epithelial ovarian cancer patients.