Study On Biocompatibility Of PCL-Gel-10wt%AgNPs Electrospinning Dressing

Objective: In this study,the biocompatibility of PCL-Gel-10wt% Ag NPs electrospinning nanofiber dressing was tested to provide guarantee for the safe application of PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing in clinical practice,and provides new ideas for comprehensive testing of the biocompatibility of new wound dressings.Methods:1.Electrospinning technology was used to prepare PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing,and the physical structure was observed under an electron microscope.2.In vitro toxicity test: Human dermal fiber cells were inoculated into a culture plate containing glass slides,a culture plate containing nanofiber membrane of PCL-Gel electrospinning,and a culture plate containing nanofiber membrane of PCL-Gel-10wt%AgNPs electrospinning,respectively.And the proliferation,adhesion,apoptosis and cell cycle differences between different groups of human dermal fiber cells were detected by CCK-8,Annexin-V analysis and cytometric assay.3.In vivo toxicity test: Kunming mouse wound model was established,the immune of mice and systemic toxicity risk of PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing on mice were determined with skin stimulation test and systemic acute toxicity test.Results:1.The structure of PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing was observed under electron microscope.The inner layer was a mixture of polycaprolactone and gelatin(polycaprolactone: gelatin =1:1),and the middle layer was a mixture of polycaprolactone and gelatin(polycaprolactone: gelatin =1:1)containing 10% mass fraction Ag NPs.The outer layer was polycaprolactone.The structure of the three layers was clear and discernible under electron microscope.2.In vitro toxicity test: According to CCK-8 results,the human dermal fibroblasts proliferation ability of p PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing group was weaker than that of 12-hole slide group and PCL-Gel electrospinning nanofiber membrane dressing group at D1,D3 and D5 after inoculation.Cell adhesion test shows that at 4h and 12 h after inoculation,the adhesion ability of human dermal fibroblasts in the PCL-Gel-10wt% Ag NPs electrospun nanofiber membrane dressing group was weaker than that in the 12-well slide group.The apoptosis test results showed that there was no statistical difference in the survival rate of human dermal fibroblasts in the PCL-Gel-10wt% Ag NPs electrospun nanofiber membrane dressing group,12-well slide group and PCL-Gel electrospun nanofiber membrane group at 48 h and 96 h after inoculation.Cell cycle test results in vaccinated 48 h and 96 h after inoculation of three groups of human dermal fibroblasts culture plate cell proliferation ability there was no significant difference,but inoculated after 48 h and 96 h PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing group of human dermal fibroblasts S phase cells ratio higher than the rest of the two groups(P<0.05).At 96 h after inoculation,the proportion of human dermal fibroblasts at G2 stage in the PCL-Gel-10wt% Ag NPs electrospinning nanofiber membrane dressing group was lower than that in the other two groups(P<0.001).3.In the in vivo cell experiment,the skin irritation test results showed that the wounds of the PCL-Gel-10wt% Ag NPs electrospinning nanofiber dressing group and the blank group did not have skin erythema,edema,local hyperemia,swelling and necrosis at 24 hours,48 hours,3 days,5 days,and 7 days after wound modeling.In the systemic acute toxicity experiment,the general behavior and activities of the mice in the PCL-Gel-10wt% Ag NPs electrospinning nanofiber dressing group and the blank group were normal within 7 days after modeling.The weight gain of the two groups of mice was within the normal range,and the difference between the two groups was not statistically significant.Conclusion:1.Electrospinning technology was used to prepare PCL-Gel-10wt%Ag NPs electrospinning nanofiber dressing,and a three-layer structure was observed under an electron microscope.2.The PCL-Gel-10wt%Ag NPs electrospun nanofiber dressing has good biocompatibility.