| Purpose:By detecting the expression of epithelial-mesenchymal transition(EMT)markers[epithelial cadherin(E-cad),neural cadherin(N-cad),β-catenin] and Wnt/β-catenin signaling pathway inhibitory protein Chibby in the eutopic and ectopic endometrium of patients with adenomyosis(AM),analyze the relationship between EMT and Chibby,discuss the role of EMT and Chibby in the occurrence and development of AM,to provide the experimental evidence and new ideas for the diagnosis and treatment of AM.Materials and Methods:1.Object of studyA total of 36 patients who were clinical diagnosed as AM and underwent total hysterectomy in the Department of Gynecology of Guangzhou First People’s Hospital from May 2019 to May 2020 were collected as the study population,and AM was confirmed by pathological examination after the operation.The study components who met the criteria for inclusion were divided into ectopic endometrium group and eutopic endometrium group.Among them,the ectopic endometrium group was endometrium tissue ectopic to myometrium,and the eutopic endometrium group was endometrial tissue located in the uterine cavity.At the same period,20 patients with CIN combined with HPV16 or 18 positive Ⅲwho insisted on total hysterectomy were collected as the control group.The normal endometrium was performed by pathologically confirmed after the operation,and the endometrial was taken as the control endometrial group.All patients had not taken hormonal drugs,not been pregnant,not breastfed,not placed the intrauterine devices within three months before the operation,meanwhile,without any other endocrine diseases,autoimmune diseases,or malignant tumors;the study group excluded the patients complicated with endometriosis and the control group excluded the patients with AM or endometriosis.2.Experimental methodImmunohistochemical technique was used to detect the expression of E-cad,N-cad,β-catenin and Chibby in the ectopic endometrium,eutopic endometrium and control endometrium.Analyzed the difference and correlation of expression in the above-mentioned tissues.3.Statistical analysisIn this study,the SPSS 25.0 software package was used for statistical analysis of the data.The measurement data was expressed as mean ± standard deviation,and the count data was expressed as percentage(%).The measurement data shall be tested for homogeneity of variance first,and those with uniform variance shall be tested by two-sample t test.Counting data was compared using chi-square test to compare differences between groups.Correlation between count data was analyzed using Spearman rank correlation analysis.Inspection level α=0.05(both sides).Result:1.The positive expression rates of E-cad in the ectopic endometrium group,eutopic endometrium group and control endometrium group were 16.7%,22.2% and85.0%,respectively.The expression of E-cad in ectopic endometrium group was lower than that of eutopic endometrium group,but the difference was not statistically significant(P>0.05).The expression of E-cad in ectopic endometrium group and eutopic endometrium group was significantly lower than that of the control endometrium group,and the differences between the groups were statistically significant(P<0.05).2.The positive expression rates of N-cad in the ectopic endometrium group,eutopic endometrium group and control endometrium group were 69.4%,44.4% and10.0%,respectively.The expression level of N-cad in the ectopic endometrium group and the eutopic endometrium group was significantly higher than that of the control endometrium group,and the differences between the groups were statistically significant(P<0.05).The expression of N-cad in the ectopic endometrium group was significantly higher than that in the eutopic endometrium group,and the difference was statistically significant(P<0.05).3.The positive expression rates of β-catenin in the ectopic endometrium group,eutopic endometrium group and control endometrium group were 72.2%,41.7% and10.0%,respectively.The ectopic expression level of β-catenin in the ectopic endometrium group and the eutopic endometrium group was significantly higher than that of the control endometrium group,and the differences between the groups were statistically significant(P<0.05).The ectopic expression level of β-catenin in the ectopic expression group was significantly higher than that in the eutopic endometrium group,the difference was statistically significant(P<0.05).4.The positive expression rates of Chibby in the ectopic endometrium group,the eutopic endometrium group and the control endometrium group were 66.7%,88.9%and 95.0%,respectively.The expression level of Chibby in the eutopic endometrium group was lower than that of the control endometrium group,but the difference was not statistically significant(P>0.05);the expression level of Chibby in the ectopic endometrium group was significantly lower than that in the eutopic endometrium group and the control endometrium group,the differences between the groups were statistically significant(P<0.05).5.In the ectopic endometrium,Chibby is negatively correlated with β-catenin(r=-0.470,P<0.05),and has no correlation with E-cad and N-cad(P>0.05);in the eutopic endometrium,Chibby has no correlation with E-cad,N-cad,β-catenin(P>0.05);in the normal control endometrium,Chibby was positively correlated with E-cad(r=0.545,P<0.05)and negatively correlated with β-catenin(r=-0.489,P<0.05),while having no correlation with N-cad(P>0.05).Conclusion:The expression levels of E-cad and Chibby in the ectopic endometrium group were significantly lower than those in the control endometrium group,while β-catenin and N-cad in the ectopic endometrium group were significantly higher than those in the eutopic endometrium group and the control endometrium group,suggesting that AM occurred EMT and Chibby’s expression in AM decreased.In the ectopic endometrium and the control endometrium,Chibby was negatively correlated withβ-catenin;in the control endometrium,Chibby was positively correlated with E-cad,suggesting that EMT and Wnt/β-catenin signaling pathway inhibitory protein Chibby may be relevant in the occurrence and development of AM. |
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